Purpose: Examine the role of the cell membrane in the cell by disrupting its function using temperature (Biology 107 Laboratory Manual 2014). This will improve the general understanding of optimal growing temperatures and the breakdown of the cell membrane Procedure: Betacyanin solution of a known concentration was diluted to create a dilution series‚ then placed in a spectrophotometer set to 525 nm. The absorbance of the dilution was used to create a standard curve for betacyanin. Discs of living
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Practical 2.1- The Effect of Temperature on Membranes Objective To investigate the effect of temperature on membrane structure Introduction Beetroot Pigments Beetroots contain Betalains which are the red pigments present in the cell vacuole. Betalains are soluble in water and they contain nitrogen. Betalains extracted from beetroot is commonly used as food dye because it is not known to cause any allergic reactions. Beetroot Picture taken from http://tipdeck/how-to-cook-beet-root Structure
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of the erythrocyte‚ were combined with a 0.2mL of well-mixed stock suspension were added to 0.27M of each solution‚ one at a time‚ and timed how long it took for hemolysis to occur with a stop watch. As soon as the blood was introduced into the solvent‚ we shook the mixture to ensure the process would happen correctly and efficiently. Results Table I gives the values in seconds of how long all 11 solutes took to hemolysis in seconds. Each molecule has been tested by 7 separate groups as to
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ORGANIC MATTER AND INORGANIC MATTE Organic matter is that which is living or has been alive‚ e.g. wood or coal. Inorganic matter is that which is not living and never has been alive‚ e.g. water or iron. Inorganic substances can be built up into organic compounds by living organisms. All living matter is made up of small units called cells. Some organisms‚ such as bacteria‚ consist of one single cell; others‚ like human beings‚ consist of many hundreds of millions of cells‚ all functioning together
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occur. 3) Which of the following statements about carrier proteins is FALSE? 3) _______ A) They can become saturated if the maximum transport rate is exceeded. B) They are found integrated into the plasma membrane. C) They assist in simple diffusion. D) They might have to change shape slightly to accommodate a solute. 4) Osmotic pressure is measured in units of _______. 4) _______ A) mm Hg B)
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the effect of different concentrations of ethanol on the permeability of beetroot cell membranes. Prediction: By exposing a membrane to a solvent‚ ethanol‚ it will increase its permeability. So the higher the concentration of the solvent‚ the more permeable the membrane will be. But if the concentration of the ethanol is beyond a certain limit‚ it may break down the cell membrane to the point where there is in fact no overall effect as the ethanol would disturb the lipids in the membrane.
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chemicals that give it strength‚ flexibility‚ and resistance to chipping and peeling. WHAT IS IT MADE OF? 1. First‚ solvents are used to mix together all the ingredients in a nail polish to yield a uniform liquid product. a. The type and amount of solvent determines how thick a polish is and how long it takes to dry b. Most Common solvent- ethyl acetate. c. Ill talk more about what the solvent does in a few slides. d. See pic 2. Another ingredient are resins- a polymer material that holds the polish
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Introduction To determine the effects of stressful experimental treatments on living membranes we are going to examine how fresh beets roots react when they are exposed to different temperatures. Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell 133). They organize specific chemicals and reactions into specific compartments within the cell. Generally‚ cell membranes consist of phospholipids bilayer
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stress that various alcohols have on biological membranes. Using five solutions of differing alcohol concentration for each of the three alcohols; methanol‚ ethanol‚ and 1-propanol and a small slice of beet‚ the stirred solution was placed into a plastic cuvette and then into a spectrophotometer and the absorbance of alcohol solutions were determined in order to conclude which alcohol and concentration of alcohol had the greatest effect on biological membranes. The results showed that the most non-polar
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Investigate factors affecting cell membrane permeability in beetroot. Research Question: How does variation in temperature affect the permeability of cell membranes? Background Information: In this investigation I will want to identify how variations in temperature effect the permeability through the use of a beetroot. I predict that as the temperature of the water increases so will the dispersion of the pigment. Independent Variable: The temperature of water will be increased in increments of 10oC
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