DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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This lab must be typed. Title DNA Fingerprinting Purpose Why are we doing this lab Background 1. What are restriction enzymes 2. When added to a DNA sample‚ what do restriction enzymes do 3. What do you call the specific sequence of bases the enzyme is searching for 4. What is a restriction digestion 5. What is the purpose of the water bath 6. The electrophoresis apparatus creates an electrical field with positive and negative poles at the ends of the gel. DNA molecules are negatively charged.
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The history of DNA structure discovery(sec.4.1): 1869- Johan miescher •studies the nuclei of white blood cells(isolated th material using HCL and digestive proteins •Named the substance nulclein and also found the material was rich in nitrogen and phosphorus. 1919-Pheobus levene •Discovered that DNA was made of chains of nucleotides *see nucleotide structure* 1920 DNAvsRNA * see chart •Thought that 4 nucleotides were connected in the same repeated pattern •protein gas 20 amino acids
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DNA‚ RNA‚ PROTEINS STARTS WITH ? Name _______________________________ 1. DNA that is spread out in the nucleus of a non-dividing cell so it can be read is called _C_ __ __ __ __ __ __ __ __ . 2. The group of 3 nitrogen bases in the mRNA message that is read together is called a _C_ __ __ __ __. 3. In dividing cells‚ the DNA is scrunched into _C_ __ __ __ __ __ __ __ __ __ __ so it can be moved. 4. The mRNA message tells the ribosomes which _A_ __ __ __ __ _A_ __ __ __ to put in next
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DNA Transcription The process of transcription is where a copy of a gene is made within DNA to use as RNA. It is located in the nucleus of eukaryotes and in the nucleoid of prokaryotes. DNA stores information encoded in a genetic code. The code consists of four letters and they are T (thymine)‚ G (guanine)‚ A (Adenine)‚ and C (cytosine). One gene codes for on protein. RNA is a molecule that copies information that is coded in another genetic code. This code also consists of the same four
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Harvard University Researchers Develop Cloaked DNA Devices for Medicine and Treatments By John Nassivera | Apr 29‚ 2014 05:20 PM EDT Researchers from Harvard University’s Wyss Institute for Biology Inspired Engineering have created a cloaked DNA nanodevice that can avoid defenses in the body’s immune system. The technology’s design was given inspiration from world viruses‚ according to Gizmag. The nanoscale device could be used for diagnosing
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. DNA molecules are composed of two strands that form a helical ascending spiral. They fit together like the opposing teeth of a zipper and are held together by weak interactions called hydrogen bonds. These two strands is a long string of subunits called nucleotides‚ each attached to the one immediately about it and the one immediately below it to form a long chain. Each
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Criminal Profiling 2 Abstract All societies in the world are troubled by crime everyday. The general public has become very fascinated by criminals and fearful of criminal behavior. In the fight against crime‚ criminal profiling has been developed to aid the FBI in the capture of criminals. At the heart of criminal profiling is a combination of psychological principles and crime scene analysis. In combining both the psychological principles and crime scene analysis‚ it is possible to identify
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least 100 words: 1. Describe the structure of DNA. DNA is a structure of specific molecules and a complex mixture of chemicals. DNA is a nucleic acid‚ which is a group of complex compounds that can be found in all living cells or viruses‚ and controls cell health and function. Nucleic acids are composed of polymers and monomers‚ which are referred to as nucleotides. There are four different types of nucleotides that make up the structure of DNA‚ which are abbreviated A‚ C‚ T‚ and G. Covalent
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Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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