ABSTRACT Antibacterial activity of medicnal plants has turned out to be a global concern. Medicnal plants are very useful in treating various diseases. Antibiotics are an important class of pharmacological agents used for treating antibacterial infections. Although antibiotics were first isolated from fungi and bacteria but over the years more and more synthetic antibiotics has been synthesized‚ which are in great demand. This present study has been done to determine the antibacterial activity of
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Experiment 22 Laboratory to Biology III Diversity of Microorganisms / Wintersemester / page 1 Experiment 22 Isolation of plasmid-DNA from bacteria and PCR Advisor Konrad Egli: kegli@botinst.unizh.ch Reading Chapters in BBOM 10th: 10.8 BBOM : Madigan M.T.‚ J.M. Martinko and J. Parker: "Brock - Biology of Microorganisms"‚ 10th Edition (2003)‚ Prentice Hall. Objectives Background • Isolation of plasmid-DNA from different bacteria clones • Handling of bacteria clones
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Cobalt(II) Oxygen adduct complex Inorganic 461 lab 2-29-2012 Abstract This experiment uses Co(salen) as a model compound illustrating the uptake of oxygen in hemoglobin. SalenH2 was prepared as the intermediate‚ and reacted with hydrated cobalt to prepare Co(salen). Using Co(salen) the oxygen up-take was tested. The synthesis of SalenH2 produced 0.65 g. The percent yield of salenH2 was 116% on the filter paper and 105% after transferring salenH2 to a vial. The melting point of salenH2‚ was
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independent variables by changing the amounts added and the order in which they were added to test what chemicals and what order they needed to be added to the reaction to produce a glow. Results: Trial Number | Luminol | Bleach | DMSO | 1M NaOH | 1M HCL | H2O2 | Results | A1 | Tad 1 | 1D 2 | 1D 6 S | 1D 5 | 1D 4 | 1D 3 | Turned brown | A2 | Tad 1 | 7D
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Drugs and chemicals Sitagliptin (Sita) was manufactured by Merck Sharp & Dohme Ltd (Pavia‚ Italy)‚ dissolved in saline. Gentamicin sulfate was obtained as ampoules from Memphis Co.‚ for Pharm. and Chem. Ind. (Cairo‚ Egypt). Other used chemicals were purchased from Sigma Chemical Co. (St. Louis‚ MO). Animals Animal procedures and care were in accordance with the National Institute of Health guide for the care and use of Laboratory Animals (NIH Publication No. 85-23‚ revised 1996) and were approved
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of Harvoni is a combination of Ledipasvir and Sofosbuvir. Ledipasvir is a white to tinted‚ slightly hygroscopic crystalline solid which is Slightly solube in pH 2.3 buffer but partially insoluble in pH 4-7.5 buffers. This is Soluble in ethanol and DMSO but slightly soluble in acetone. Sofosbuvir is a white to off-white non-hygroscopic crystalline solid which is slightly soluble in water (pH1.2-7.7). this is also soluble in ethanol‚ acetone‚ 2-propanol.[4] 3.2 Drug Product: HARVONI is a fixed-dose
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know the elements of life – and how the reduction and oxidative of some of these drive bioenergetics oxidize carbon (i.e. glucose source) and reduce oxygen (electron acceptor) (CHNOPS) and iron (Fe; trace element; Fe also important to obtain cellular energy). Nutrition: CHNOPS Trace Elements: Iron (Fe)‚ Ni‚ Mg‚ Mo‚ Mn‚ Ca (important in humans due to osteoblastic activity)‚ Co Occupancy: beat out others for a spot to live‚ e.g. microbiota (normal flora) Resistance: defend against microbes‚ eukaryotes
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The house mouse (Mus musculus) is a small rodent‚ a mouse‚ one of the most numerous species of the genus Mus. The house mouse has been domesticated as the pet or fancy mouse‚ and as the laboratory mouse which is one of the most important model organisms in biology and medicine. It is by far the most commonly used genetically altered laboratory mammal. Mouse and man The sequence of the mouse genome was published in 2002. "The mouse and human genomes are very similar‚" says Dr Jackson. "There
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Structural Effects on Stability and Reactivity. Organic Chemstry Laboratory Structural Effects on Stability and Reactivity Introduction The concepts of stability and reactivity are fundamental to understanding chemistry. In this chapter we consider first the thermodynamic definition of chemical stability. We then consider chemical kinetics (Section 3.2) and how it can provide information about reactivity. We also explore how structure influences stability and reactivity. We want to learn how
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Buffer Preparation (Gozani Lab) 1. 1 M Tris-HCl Buffers pH Volume (L) TrisBase (g) HCl (ml) pH 7.0 2 242.2 150-155 pH 7.5 2 242.2 120-125 pH 8.0 2 242.2 80-85 Autoclavable. 2. EDTA 0.5 M (pH8.0) 0.5M‚ 1L: 148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g EDTA-Na.2H2O + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make
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