poorest results. Material and Methods: 1. Get 4 deep petri dishes and label them 1‚ 2‚ 3‚ and 4. Fill dish 1 about 2/3 full with distilled water. Fill the other 3 about 2/3 full with 0.2% NaHCO3 solution. Set these aside for later. 2. Pour about 100 ml of 0.2% NaHCO3 solution into a flask with a side arm. 3. Get three or four spinach leaves and cut 40-50 disks with a cork borer. Do not include the large veins in the disks that you make. Stack the leaves so you can cut through them all and cut
Free Carbon dioxide Photosynthesis Oxygen
Enzyme B produced the most maltose at a high temperature (100 ºC) and an acidic pH. Enzyme C produced the most maltose at 37 ºC (body temperature) and a pH of 7 (neutral). Conclusion In a prior lab‚ it was given that the enzyme Aspergillus oryzae (fungus) works best at warm temperatures and an acidic pH‚ porcine (pig) works best at body temperature (37 ºC) and
Premium Enzyme Chemical reaction Metabolism
Ph Lab Report Bryon Kim 123013 B(2) Biology Background information/Research PH paper (litmus paper) determines how acidic or how basic a substance is. The paper changes color accordingly to color code on the pH scale. The pH scale starts from 0 to 14. The lower the number the more acidic it is. Zero is the most acidic‚ and 14 is the most basic while 7 is the neutral number for example water. Examples of an acid is lemon juice or multi purpose cleaner. Examples of a basic substance is shampoo or liquid soap
Premium Green Purple Blue
Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential
Premium Gram staining Staining Microbiology
Photosynthesis and the Amount of Light _______________________________________________________________________ I. Introduction Photosynthesis the process where plants use sunlight (energy) to synthesize foods forming the products carbohydrates and water (H20 + CO2 + Light CH2O + O2). For photosynthesis to take place they need water‚ carbon dioxide and light and chloroplasts. Light is absorbed inside he thylakoid
Premium Photosynthesis Plant Oxygen
For the preparation of the filtrate solution at the beginning of the class our Instructor‚ Marshall Douglas added about four to five strawberries in the blender along with 100 ml of detergent/ enzyme/ salt solution. They were blended until thoroughly homogenized if foaming occurred more detergent solution was added to prevent it. Then the blended solution was poured into a beaker and left to incubate at 60C for 15 minutes. Also‚ the mixture solution was cooled on ice for 5 minutes and filter through
Premium
University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
Premium Protein Chromatography Analytical chemistry
Background The melting point of a compound is the temperature at which the solid is in equilibrium with its liquid. A solid compounds changes to a liquid when the molecules acquire enough energy to overcome the forces holding them together. A mixture melting point is useful in confirming and used to indentify an unknown compound‚ which we will be doing in this particular experiment. Purpose Measure the melting points of pure benzoic acid and pure mandelic acid. Determine the eutectic composition
Premium Chemical compound Liquid Temperature
AP Biology Lab: Catalase (Enzymes) Abstract In this laboratory exercise‚ studies of enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme
Premium Enzyme
Transpiration Formal Lab Report By Jessica Tran Introduction When water is transported from the roots to the mesophyll cells in the leaves‚ it is evaporates out the stomates‚ called transpiration‚ to create a lower osmotic potential. Osmotic potential is the part of the water potential of a tissue that results from the presence of solute particles. Even though the stomates open to release water‚ it also brings in carbon dioxide to produce sugar and oxygen through a process of photosynthesis
Premium Plant physiology Osmosis Xylem