Affect of pH on Porcine Pancreatic Alpha-Amylase Activity Introduction Proteins function in a variety of different ways‚ and one of their fundamental tasks is to act as enzymes. Enzymes are extremely important in controlling reaction speed (by initiating and regulating biological activity)‚ cell communication‚ and growth. One particularly significant enzyme is amylase‚ which catalyzes the hydrolysis of alpha glycosidic linkages of amylose‚ starch components‚ and other oligosaccharides (Qian
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Enzymatic Activity of Salivary Amylase Abstract: Salivary amylase is an enzyme that can digest starch molecules and break them down to sugar molecules. In this experiment‚ the enzymatic activity and specificity of salivary amylase was examined depending on the changes in pH and temperature. In the first part of the experiment‚ the effect of temperature was determined‚ using constant temperature bath (4‚ room temp‚ 37‚ 50‚ 60‚ and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite
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Biology Lab Report Research Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case‚ amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example‚ may taste sweet to us‚ because they contain starch. The optimum pH for pancreatic
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How does pH affect the activity of the Amylase? Abstract: In my experiment I aimed to observe how ranging pH levels will affect the rate in which amylase will break down the starch molecules. I will be measuring the time it takes for the dark liquid to disappear and leave a yellow brown liquid to be shown‚ which would show that there is no starch present in the solution because it would have broken into maltose by adding amylase. Results did not fully demonstrate what we expected in our hypothesis
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Charlene Villanueva - Biology Charlene Villanueva - Biology Starch‚ Glycogen and Cellulose Independent Research A polysaccharide is a long chain of monosaccharide molecules‚ held by glycosidic bonds. They are usually not sweet in taste‚ insoluble in water and often do not produce crystals when water is taken out. Starch Starch is a complex solid carbohydrate‚ consisting glucose molecules held together by glycosidic bonds. It is a storage polysaccharide. They can be found in fruits‚ seeds‚ roots
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experiment was to determine the effect of temperature on the rate of movement of Porcellio scaber. Our prediction was that P. scaber would have faster rates of movement in hotter temperatures. We expected this response as P. scaber is an ectotherm. Hence‚ its body temperature is directly determined by the temperature of its environment (Mellanby 1939). Løkke and van Gestel (1998) stated that the rate of the metabolism is controlled by the environmental temperature for ectotherms. As seen by Wieser‚ cited
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Enzymatic Activity of Salivary Amylase Ong‚ Janela Rose I.; Paguia‚ Maria Tricia C.; Placente‚ Dax Daven A.; Posadas‚ Grace Catherine A. 3Bio3-Group 8 Department of Biological Sciences‚ College of Science University of Santo Tomas‚ España‚ Manila 1008 Abstract This experiment aims to examine the enzymatic activity and specificity of salivary amylase depending on the changes in pH and temperature; and determine the optimum temperature and pH of the amylase. EXPERIMENTAL In the
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meter (N/m2)‚ or pascals (Pa). 3. Slowly drag the temperature (T) slider back and forth. (Note: In this Gizmo‚ the Kelvin scale is used to measure temperature. On the Kelvin scale‚ 0 degrees is absolute zero‚ the coldest possible temperature. Absolute zero is equal to -273.15 °C or -459.67 °F) A. How does the change in temperature affect the speed of the molecules? __If the temperature is colder the molecules move slower and if the temperature is hotter the molecules move faster.
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Abstract α-amylase was immobilized covalently on iron oxide magnetic nanoparticles. The synthesis of magnetic nanoparticles was done by the coprecipitation conventional method. The chemical composition and particle size of the synthesized particles was confirmed via X-ray diffraction. Tyrosine‚ Lucien and chitosan and glutaraldehyde were investigated to make a covalent binding between the iron oxide magnetic core and the immobilized enzyme. Immobilization using chitosan and glutaraldehyde show
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To begin the analysis‚ the properties of the air at the base of the tower were examined. The wet and dry bulb temperatures of the air at the inlet were used to find the humidity‚ humid volume‚ and enthalpy of dry air. For example‚ in the first trial the dry bulb temperature was 22.5⁰C and the wet bulb was 16.5⁰C‚ and the properties found from the psychrometric chart are listed below. H_bottom=0.0095 kg/(kg dry air) V_bottom=0.85 m^3/(kg dry air) h_bottom=47 kg/(kg dry air) Then‚ the cross-sectional
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