The reaction between alpha-amino acid and ninhydrin involved in the development of color are described by the following five mechanistic steps: alpha-amino acid + ninhydrin ---> reduced ninhydrin + alpha-amino acid + H2O alpha-amino acid + H2O ---> alpha-keto acid +NH3 alpha-keto acid + NH3 ---> aldehyde + CO2 Step (1) is an oxidative deamination reaction that removes two hydrogen from the alpha-amino acid to yield an alpha-imino acid. Simultaneously‚ the original ninhydrin is reduced
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identify an unknown amino acid. IntroductionAmino acids are simple monomers which are strung together to form proteins. Amino acids play a key cellular role in structure and function. Proteins themselves participate in nearly every physiological event in the cell. Since all amino acids contain at least one amino and one carboxyl group‚ they are classified as amphoteric substances (meaning that they can act as either an acid or as a base) (1). Treating the zwitterion with acid will result an addition
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for Intact proteins and protein hydrlysates (at least tripeptide‚ 2 peptide bonds Result Pink to violet blue color Ninhyrin Test 1‚2‚3-indanetrione monohydrate or triketohydintene hydrate‚ ethanol Alpha- amino group (usually a general test for amino acids) Xanthoproteic Test Conc. HNO3‚ conc. NaOH For W‚F‚Y (aromatic except for H) Blue to blue-violet Oxidative decarboxylation color & deamination followed by (proline:hydroxypr condensation oline gives a yellow
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There are three different types of epithelial tissues that are found inside the human body. There is the cutaneous membrane (tissue)‚ which we would refer to as “skin”. This part is the only epithelial tissue that is touched by air and is dry. The cutaneous membrane is composed of keratinizing stratified squamous epithelium. The second is the mucous membrane‚ which is the tissue used for absorption and secretion‚ protective lubricating mucus in the repertory and digestive system. This membrane
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The Mouse does not know life until it has been into the mouth of a cat. The Vietnamese people are an ethnic group originating from present day Northern Vietnam and Southern China. Although geographically and linguistically labeled as Southern Asians‚ long periods of Chinese domination and influence have placed the Vietnamese culturally closer to East Asians. The Vietnamese people culturally believe in myths and the supernatural. According to the Thai and Vietnamese Proverbs and common expressions
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(Cooper‚ 139)" (Protas‚ Allison). "River symbolism is based around water in movement" (Fraim‚ John). "On the river Huck and Jim witness life and death‚ tragedy and comedy‚ strife and peace" (The Adventures of Huckleberry Finn). In this case‚ the river ha... ... middle of paper ... ...her authors use symbolism in their own personal novels‚ one can see that Twain’s symbolism dramatically stands out. It is not difficult to point out the outstanding symbols Twain chose to represent in this all-time
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the RNA bases that are complementary to the DNA template strand that uses uracil opposite to adenine. The RNA polymerase which is an enzyme that moves from the 3’ to 5’end on DNA template strand to synthesis mRNA from 5’ to 3’. b. What is the amino acid sequence produced by translation of the mRNA sequence?
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Ovarian cancer can develop from the epithelial cells surrounding the ovary‚ the germ cells‚ or stromal cells within it. Epithelial ovarian carcinomas account for 85-90% of ovarian cancers‚ and will be the focus of this essay. Most symptoms of epithelial ovarian cancer are due to the fact that it often spreads to the lining of and organs in the abdominal cavity. These symptoms include bloating‚ pelvic or abdominal pain‚ urinary urgency or frequency‚ and trouble eating associated with feeling of fullness
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CHAPTER 5. DISCUSSION 5.1. Water quality The average water temperature and average dissolved oxygen obtained during the experiment has been reported as the optimum range (3-10mg/l) for tilapia growth and yields (Meske‚ 1985). Siddiqui et al. (1989) stated that Oreochromis mossambicus has a low oxygen demand and can survive at low oxygen levels (1-2mg/l). Riche and Garling (2003) reported that dissolved oxygen levels should be maintained above 4.9 mg/l for best growth. The water quality tolerance
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Classification of Epithelial Tissue Location of Epithelium The four basic tissue types in the body are the epithelial‚ connective‚ muscular‚ and nervous tissue. These tissues exist and function in close association with one another. The epithelial tissue‚ or epithelium‚ consists of sheets of cells that cover the external surfaces of the body‚ line the internal cavities‚ form various organs and glands‚ and line their ducts. Epithelial cells are in contact with each other‚ either in a single
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