Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
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the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic or basic‚ the catecholase was less effective
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MEASURING pH Date of Lab: 02/09/13 Date Report submitted: 02/26/13 Purpose of the experiment By doing this experiment‚ we were trying to learn how to measure a pH with a pH meter to determine whether a solution is weakly‚ moderately or strongly acidic or basic; but also to compare the different data founded to see how the addition of certain liquid to a solution may affect the pH of the initial solution. Materials We used: * Tap water‚ distilled water‚ Soda and milk as the main
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The pH Scale is a scale made to show how acidic or alkaline a substance is. The pH of something is measured with pH paper which can vary from wide range paper‚ which covers all ph’s‚ but is not as accurate at some others. There are pH papers that get very specific that can have a range such as 2 or 3 and get much more precise. The neutral on the scale is 7. Anything that is below 7 is considered acidic and anything above 7 is considered alkaline. Acidic substances contain more H+ than OH-. H+
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1 Lab Report Title: Measuring pH Levels Instructions: Enter the Virtual Lab‚ and conduct the experiments provided before going out into the virtual field for additional research. Please type your answers on this form. When your lab report is complete‚ submit it to the Submitted Assignments area of the Virtual Classroom. Part I: Answer the following questions while in the Phase 1 lab environment. Section 1: You will be testing 4 known solutions for pH levels using a standard wide-range
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Experiment 20: pH Titration: Phosphoric Acid in Cola Drinks Post-lab Assignment or Report The post-lab report for this experiment is due at the beginning of the following lab period. Student notes for the lab will be available on the lab T-Square site. Learning Objectives Students will be able to... • Use a known mass of solid acid to determine an unknown concentration of a basic solution (this process is called “standardization”). • Execute a titration using good‚ reliable technique.
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PH at the UN Alfredo Vidal Ceballos 7/10/2014 Group Support Part 1: Solution Group Name and M. cons. Chem formula Type Experimental pH 1 Deidre Acetic Acid 0.1M HC2H3O2 Weak Acid 3.03 3.03 2 Acetic Acid 1M HC2H3O2 Weak Acid 2.59 2.54 3 Hydrochloric Acid 0.5M HClStrong Base 0.85 0.90 4 Jonah Nitric Acid 0.1M HNO3 Strong Acid 2.14 2.14 5 Sodium Acetate 1M NaC2H3O2 Weak Base 9.42 9.42 6 Potassium Dihydrogen Phosphate 0.1M KH2PO4 Weak Acid 5.47 5.47 7 Andy Potassium Hydrogen Phosphate 0
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pH pH means Power of Hydrogen or Potential of Hydrogen. pH is a scale of acidity from 0 to 14. It tells how acidic or alkaline a substance is. More acidic solutions have lower pH. More alkaline solutions have higher pH. Substances which are not acidic or alkaline i.e neutral usually have a pH of around 7. Acids have a pH less than 7. Alkalis have a pH greater than 7. There are a lot of ways for finding the pH of something. One way is to use litmus paper. The pH paper is able to tell how strong
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Lab Report: Purpose: The Objective of this lab was to learn how to measure the pH (or acidity) of commonly known fluids‚ using the correct tools and procedures. To then use that data to document the changes noticed when mixing those same fluids and changing their respective pH levels. Materials: In order to conduct this experiment several pieces of equipment and other materials were needed. The first item was a graduated cylinder‚ which was used in order to measure out the precise
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Question 1 Critique Dr. Honeydew’s report. Specifically‚ discuss what is missing and how the missing information should have been presented. First the PH measurements‚ Dr. honey dew does not explain how the PH device was calibrated or if the blood sample was added or mixed with anything prior to the measuring of the pH (like water‚ etc.). In regards to the absorption spectroscopy‚ the method by which the concentration was obtained is questionable. Was the concentration known before the experiment
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