University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Background The melting point of a compound is the temperature at which the solid is in equilibrium with its liquid. A solid compounds changes to a liquid when the molecules acquire enough energy to overcome the forces holding them together. A mixture melting point is useful in confirming and used to indentify an unknown compound‚ which we will be doing in this particular experiment. Purpose Measure the melting points of pure benzoic acid and pure mandelic acid. Determine the eutectic composition
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AP Biology Lab: Catalase (Enzymes) Abstract In this laboratory exercise‚ studies of enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase from starch and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme
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Transpiration Formal Lab Report By Jessica Tran Introduction When water is transported from the roots to the mesophyll cells in the leaves‚ it is evaporates out the stomates‚ called transpiration‚ to create a lower osmotic potential. Osmotic potential is the part of the water potential of a tissue that results from the presence of solute particles. Even though the stomates open to release water‚ it also brings in carbon dioxide to produce sugar and oxygen through a process of photosynthesis
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graduated cylinder‚ 50 mL graduated cylinder‚ small and large test tube‚ 50 mL and 150 mL beakers‚ 50 mL Erlenmeyer flask‚ digital top-loading balance‚ unknown liquids for density determination‚ irregular solids for density determination‚ safety glasses‚ Lab manual that was used for measurement. Introduction The International System of Measurement (SI) is used worldwide and has been adopted as the official system of measurement by most countries. It is commonly called the metric system. Our traditional
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was higher than the actual melting point of pure aspirin; 135°C. The phenol test showed that the product is not a pure aspirin by expressing purple color. TLC results weren’t high enough to conclude that the product was a pure aspirin. Overall‚ the lab wasn’t successful due to the low percentage of aspirin that were produced. Out of 2.0 grams of aspirin‚ only 0.7862 grams of pure aspirin were produced. So the percent yield was only 39%. Errors could be made during filtration process and the melting
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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necessary to produce a temperature change of 1°C per gram of substance. The specific heats of different substances vary‚ and therefore this quantity may be useful in identifying an unknown. The measurement of heat changes is called calorimetry. In this lab activity‚ calorimetry will be used to determine the specific heat of an unknown metal. This will be done by using a styrofoam cup calorimeter containing water. A calorimeter is insulated so as to minimize any loss of energy to the surroundings. Therefore
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Instructor Biology 1111 4-5 Lab Topic 4: Microscopy Elodea Cells at ___X Elodea Cells at ___X Report Sheet—Lab Topic 4 1. Draw and label each of the organisms available. Cheek Cells at ___X Cheek Cells at ___X Name _______________________________ Date_____________ Instructor ___________________________ Section___________ _________________________ 4-6 Lab Topic 4: Microscopy 2. Fill in the following table: Compound Microscope Dissecting Microscope Types of Light Available Powers
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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