referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present in our 2 mL tube. Again‚ only 20 µL were used for the gel electrophoresis. Lane 3: 7 µL of a pKAN- EcoRI/HindIII double digestion. Within the double digestion
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1 Detecting glowing E.Coli Colonies by making recombinant DNA from the lux operon of Vibrio Fischeri to pGEM. Liao‚ Tffany The marine bacterium Vibrio Fischeri produced bioluminescence effect due to lux operon transcription. The purpose of the experiment is to create a genomic library of Vibrio DNA and clone the lux operon by making Recombinant DNA and transform into another organism‚ E. Coli. Chromosomal DNA of vibrio fischeri was first extracted and digested with restriction enzyme Sal I‚ then
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is the difference between traffic lights with red arrows and those with solid red lights a. You cannot turn on a red arrow‚ even if you stop first. 7. Which of the following increases your chances of having a collision? a. Continually changing lanes to pass other vehicles. 8. Which of these statements about drugs and driving is true a. Even over-the-counter drugs can impair your driving. 9. You should allow more space in front of your vehicle when following large trucks because: a. You need
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that one thing that drives someone insane and has no power over something to change it? Or have that annoying noise that constantly pops up and never goes away?Like an itch that can never be found‚ or being stuck behind someone going slow in the fast lane and they will not to let others pass. Or someone is smacking his or her gum in your face really loudly. The biggest pet peeve that others might have is that people don’t use turn signals to indicate where they are going‚ or to help prevent the cause
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audience. Scene 2: Lane tells the audience about how her maid doesn’t clean her house‚ and that she took her to the hospital to be medicated. Scene 3: Virginia tells the audience how she loves to clean her house‚ and that she believes that it is foolish to not clean your own house. Scene 4: Matilde tells the audience about how her parents always laughed‚ and how her mother died laughing at a joke and her father killed himself shortly afterwards. Scene 5: Lane tries
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using the traditional methods of television‚ print‚ and radio‚ many agencies also use a method known as out-of-home advertising. A few of the common outdoor advertising opportunities include using billboards‚ street furniture‚ and signs on transit. Lane‚ King‚ and Reichert write that the pros of using outdoor advertising include wide-spread exposure to many adults with a high frequency rate and some of the lowest costs in advertising. It also provides the freedom to use color and lighting to draw
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embroiders the cow under the shade of the tamarind tree in the middle of their courtyard. Koly sees her husband for the first time on the day of her wedding. Sensing that something is terribly wrong‚ Koly hides the silver earrings behind a loose brick in the wall‚ and claims that she has lost them. Later that night‚ she discovers that her husband is terminally ill. Sass
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SDS-PAGE analysis. 1 2 3 4 Figure 1. SDS-PAGE of purified protein X. Lane 1‚ Protein ladder (in Daltons). Lane 2‚ purified protein X (affinity chromatography). Lane 3‚ purified protein X (company manufactured). Lane 4‚ elution buffer. a. What is the benefit of a protein ladder/molecular weight marker in an SDS-PAGE gel? Describe what you can learn about the protein bands found in lanes 2‚ 3 and 4 based on the protein ladder bands. The benefit of protein ladder is to have a
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a safe neighborhood filled with young families‚ a high concentration of senior citizens‚ and people of all ages in between; however‚ when it comes to riding bicycles San Diego is completely the opposite. Riding a bicycle in areas that have no bike lanes can be a nightmare. As a former bike commuter in San Diego‚ I wanted to bring to your attention that many unreported bike accidents have occurred close to San Diego State University. While riding my bicycle to school‚ I have seen other bicyclists get
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what they wanted from Alexander. Additionally with his father’s background of military and “Hellenic Alliance” (Lane Fox‚ p.229) it is not hard to see that Alexander was immensely influenced by his father’s actions. Alexander’s values were shaped by his father. “the more a king conquered‚ the more secure his personal kingship became‚ and the more his resources for yet more conquest” (Lane Fox‚ p.230). Alexander’s
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