colored pigment called betacyanin‚ it was easy to visually see how the pigment comes out when in the presence of an alcohol. For this particular lab‚ one might have a researchable question as so: "Does ethanol effect cell membranes?" To this question many hypotheses were formed. For this personal experiment‚ if the concentration of ethanol is increased‚ then it is expected that the cell membrane of the beet root will be damaged‚ resulting in the release of more betacyanin. To test this‚ a beet
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membrane Procedure: Betacyanin solution of a known concentration was diluted to create a dilution series‚ then placed in a spectrophotometer set to 525 nm. The absorbance of the dilution was used to create a standard curve for betacyanin. Discs of living beet roots were then placed in water and exposed to various temperatures. A sample of the water that the beets were in was taken and absorbance was determined by the spectrophotometer and concentration from the standard curve for betacyanin. Findings: In
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function. Red beets cells contain central vacuoles that house a pigment called Betacyanin. When beet cells remain intact‚ Betacyanin stays within the cell. Once the beet cells are disrupted‚ betacyanin will leak out and create a pink/red color in the environment. An increase in membrane damage will cause more betacyanin leakage; hence‚ we will see a higher absorbance reading on the spectrophotometer. Thanks to betacyanin release and measuring absorbance we can conclude how much the cell membranes are
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The Effect of Solvent Exposure and Temperature on Beet Cell Membrane Integrity Introduction: • Cell membranes are vital to proper cellular function • Cell membranes consist of a lipid bilayer made up of phospholipids • Phospholipids are amphipathic molecules meaning they have a dual nature in that they show both hydrophilic (water-loving) and hydrophobic (water-fearing) properties • The amphipathic nature of phospholipids causes them to spontaneously form bilayers in water
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leaves the cell. However‚ certain conditions may damage the cell membrane’s structure. Beet cells‚ which are plant cells‚ have a water-soluble pigment called betacyanin‚ which is stored in its vacuole. The tonoplast‚ or the vacuole’s membrane‚ acts with the cell membrane of the cell to prevent the betacyanin from leaking into the cytosol or out of the cell. This lab investigates the environmental possibilities that can damage membranes. Hypertonic and hypotonic
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environment‚ as well as responsible for regulating what goes in and out of the cell though it’s protein channels and “selectively permeable” bilayer. (Campbell‚ Reece‚ Taylor & Simon‚ 2006‚ p. 79-80) When this membrane is damaged the reddish color‚ betacyanin‚ of the beet leaks out into it’s surroundings. (Online lab #1‚ n.d.) Based on previous experience I know that applying heat can act as a dilator and cold as a constrictor. If this fact should remain true for the membrane
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color intensity of leaked betacyanin will be 10. I hypothesize that tube 2 at 55° c the color intensity of leaked betacyanin will be 8. I hypothesize that tube 3 at 40° c the color intensity of leaked betacyanin will be 6. I hypothesize that tube 4 at 22° c the color intensity of leaked betacyanin will be 0. I hypothesize that tube 5 at 5° c the color intensity of leaked betacyanin will be 8. I hypothesize that tube 6 at -5° the color intensity of leaked betacyanin will be 10.
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Exploration Research Question What is the effect of different concentrations of sucrose in a solution on the mass and surface area of a beetroot (Beta Vulgaris) piece? Background The membrane consists of a phospholipid bilayer which is semi permeable. Osmosis is the passive movement of water molecules from an area of high water potential to an area of low water potential across a semi-permeable membrane. It is easy for water molecules to pass through this membrane however solutes that are big in
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permeability of beetroot Hypothesis Organic solvent and high temperature will destroy the cell membrane and make it permeable to the red pigment. The higher the concentration of the alcohol‚ the greater will be this effect. Principle Beetroot is the material used in this experiment to demonstrate the effects of high temperature and chemicals on the permeability of the cell membranes. Beetroot contains a red pigment called betalain‚ which is located in the large central vacuole of the beetroot cells.
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fatty acid composition. This means that at temperatures below this critical point no dye in the beetroot cell would be able to leak from the cell and would not change the colour of the surrounding water. The phospholipids also undergo phase changes at higher temperatures causing the membrane to be more permeable thus allowing the red pigment to move more freely into the water surrounding the beetroot. Sources of Background Information New Understanding Biology for Advanced Level Fourth addition
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