Purpose: Examine the role of the cell membrane in the cell by disrupting its function using temperature (Biology 107 Laboratory Manual 2014). This will improve the general understanding of optimal growing temperatures and the breakdown of the cell membrane Procedure: Betacyanin solution of a known concentration was diluted to create a dilution series‚ then placed in a spectrophotometer set to 525 nm. The absorbance of the dilution was used to create a standard curve for betacyanin. Discs of living
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Investigation the effect of the organic solvents on the cell membrane of the beetroot Objective: To investigate the effect of different organic solvents‚ such as alcohol and paraffin oil‚ on the cell membrane of the beetroot by using the red pigments in the beetroot as indicator. Hypothesis: Organic solvents dissolve the organic matter in the cell membrane (such as phospholipids). This destroys the cell membrane‚ and the permeability of the cell membrane is disrupted casing red pigments to leak out
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affect the cell membrane‚ thus would then releases the purple pigments out of the vacuole which causes the leakage of the purplish liquid. Background Information The outermost layer is the cell wall‚ which is present only in plant cells and is made up of a carbohydrate called cellulose and also has other protein substances embedded within it. The cell wall is a rigid layer and gives structural stability to the cell and also limits the permeability of large substances into and out of the cell. Within
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Beetroot Practical 2.8 An investigation to find out whether the raise of temperature will increase the permeability of the cell membrane: The question being answered from doing this experiment is ‘How do different temperatures affect the permeability of the plasma membrane of beetroot?’ Beetroot contains red pigments called betalains‚ located within the cell vacuole. Normally the pigments cannot pass through membranes but they leak out when the beetroot is cooked or placed in alcohol. The aim
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1) Hypertonic refers to _______. 1) _______ A) the concentration of solutes that a cell is bathed in B) a concentration of solutes that is less than the solutes inside the cell C) the concentration of solutes inside a cell D) a solution that will cause a cell to swell 2) What happens to facilitated diffusion when the protein carriers become saturated? 2) _______ A) ATP will be utilized for transport. B)
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Description To investigate whether temperature will damage and denature the permeability of plasma membrane. Preview TITLE The effect of temperature on the cell membranes of Beetroot cells. OBJECTIVES To investigate whether temperature will damage and denature the permeability of plasma membrane. HYPOTHESIS An increase in temperature will damage and denature the permeability of plasma membrane. INTRODUCTIONS Beetroot The beet (Beta vulgaris) is a plant in the Chenopodiaceae family. It
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concentrations of ethanol on the permeability of beetroot cell membranes. Prediction: By exposing a membrane to a solvent‚ ethanol‚ it will increase its permeability. So the higher the concentration of the solvent‚ the more permeable the membrane will be. But if the concentration of the ethanol is beyond a certain limit‚ it may break down the cell membrane to the point where there is in fact no overall effect as the ethanol would disturb the lipids in the membrane. Therefore‚ more of the red
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on Beetroot Membranes _Aim_ This practical will test the effect of temperature on the integrity of the membranes‚ _Introduction_ The cells of beetroot contain a pigment called betalain in their vacuoles. It is kept inside the cells by the membranes. If these membranes are damaged‚ then the betalain leaks out. The amount that leaks out can be assessed‚ as the leaked out pigment will color the water surrounding the cells. This information can be used to find out how temperature affects cell membranes
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Investigating the Effect of Temperature on the Permeability of Membranes The permeability of membranes can be altered in several ways. From previous preliminary work I know that reagents‚ for example detergents and alcohol alter the arrangement of the phospholipids in the membrane allowing substances to leak out of the cell. Temperature also affects the membrane. As membranes relay on protein molecules to allow molecules to enter and leave the cell through facilitated diffusion the temperature must
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Topic 2 – Cell membranes Reading Chapter 5 Objectives Functions of cell membranes The fluid mosaic model The lipid bilayer – phospholipids‚ glycolipids and sterols Properties of the lipid bilayer Membrane proteins – the ‘mosaic’ in the model Solute movements across membranes BIO 1140 – SLIDE 1 http://www.nikonsmallworld.com/gallery/year/2006/id/751 Functions of cell membranes – an overview Fluid mosaic model Singer & Nicolson 1972 Fluid lipid molecules in which
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