Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
Premium Glycerol Glucose Liver
LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
Premium Plasmid Transformation Escherichia coli
PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
Premium Oxygen Carbon dioxide Ratio
Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
Premium Osmosis Cell membrane Red blood cell
Wave Nature of Light Objective: The purpose of this lab is to investigate interference‚ otherwise known as the diffraction of light. A beam of light acts a wave‚ and we are able to use equations so calculate the wavelength of the light used. The diffraction of a straight edge demonstrates that light waves bend around straight edges‚ allowing light to enter an area of shadow. When waves are superposed‚ they reinforce each other when crests are in phase and cancel out when they are not in phase
Premium Diffraction Wavelength Light
chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
Premium Chlorine Chemical reaction Sodium
Lab 1- Measurements of a Table ABSTRACT: The purpose of this experiment was to determine the value of the acceleration of a free falling object and to describe the range of experimental values. Within the experiment the items used to help provide the conclusion that 2/3 or our values fell within the 9.62m/s^2- 9.78m/s^2 range was a Vernier data-collection interface‚ Logger Pro Application and the apparatus Photogate. The results found within the 30 trial periods were all precise
Premium Standard deviation Measurement Arithmetic mean
Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
Premium Ethanol Glucose Carbon dioxide
Job Safety Analysis or Job Hazard Analysis All employers have duty of care to individuals‚ staff‚ contractors or third parties in the workplace. It is also a legal requirement that organizations should document any health and safety risks that have been identified in the workplace. Equally‚ it is necessary to record the actions taken to reduce those risks What is a Job Safety Analysis or Job Hazard Analysis? One way to increase the knowledge of hazards in the workplace is to conduct
Premium Occupational safety and health
used as linear sensor Part 2 Collision cart of known mass on horizontal dynamics tracks oscillating by the means of a springs in parallel Motion sensor and photogate connected to Science workshop interface Non-linear springy objects (rubber bands) Two rectangular weights of ~0.5 kg each to change the mass of the system Procedure: The lab experiment was done in two parts. Part 1 of the experiment was done by 2 different methods. Method 1 was with a force sensor‚ springs‚ and a weight hanger in
Free Force Mass