Molecular Biology Lab Report Essay Example
Molecular Biology Lab Report
Mapping DNA using Restriction Enzymes Ava II and Pvu II to cut Bacterial DNA
Abstract
The objective of this project is to map bacterial DNA, which is derived from E. coli, using restriction endonucleases with gel electrophoresis. The DNA fragments, after cutting has occurred, are separated using agarose gel electrophoresis. The DNA fragments are placed in the gel, and an electric current is run through the matrix of the gel-like agarose. Migration of the fragments across the gel is based on the size and charge of the fragment. After the fragments have been run in the gel, they are stained with methylene blue and viewed on a light box. This allows the DNA bands to be viewed on the gel.
Introduction
This project outlines the procedure involved in mapping DNA. Prior to mapping, restriction enzymes called restriction endonucleases recognize a specific DNA sequence wherever it occurs in a DNA molecule and cut the DNA at or near the site. Each restriction enzyme is named after the species of bacteria from which it is isolated. Restricting, as it is also known, requires energy in the form of adenosine triphosphate (ATP) and involves physical cleaving of chemical bonds. The sites where cuttings occur are palindromic, that is, the sequences of the complementary strands read the same backward and forward. Naturally, restriction enzymes can recognize and metabolize foreign DNA, and this is of significant importance in bacteria as it constitutes the immune system, since it recognizes and rids it of invaders. After cutting has occurred, gel electrophoresis is used to separate the cut fragments. The gel used in the electrophoresis chamber is agarose gel which is derived from several species of red marine algae, or seaweed. It is also used in size exclusion chromatography. Agarose gel is used to separate and analyze proteins and DNA. In this experiment, agarose gel electrophoresis is used to separate the cut fragments. The DNA fragments are
Mapping DNA using Restriction Enzymes Ava II and Pvu II to cut Bacterial DNA
Abstract
The objective of this project is to map bacterial DNA, which is derived from E. coli, using restriction endonucleases with gel electrophoresis. The DNA fragments, after cutting has occurred, are separated using agarose gel electrophoresis. The DNA fragments are placed in the gel, and an electric current is run through the matrix of the gel-like agarose. Migration of the fragments across the gel is based on the size and charge of the fragment. After the fragments have been run in the gel, they are stained with methylene blue and viewed on a light box. This allows the DNA bands to be viewed on the gel.
Introduction
This project outlines the procedure involved in mapping DNA. Prior to mapping, restriction enzymes called restriction endonucleases recognize a specific DNA sequence wherever it occurs in a DNA molecule and cut the DNA at or near the site. Each restriction enzyme is named after the species of bacteria from which it is isolated. Restricting, as it is also known, requires energy in the form of adenosine triphosphate (ATP) and involves physical cleaving of chemical bonds. The sites where cuttings occur are palindromic, that is, the sequences of the complementary strands read the same backward and forward. Naturally, restriction enzymes can recognize and metabolize foreign DNA, and this is of significant importance in bacteria as it constitutes the immune system, since it recognizes and rids it of invaders. After cutting has occurred, gel electrophoresis is used to separate the cut fragments. The gel used in the electrophoresis chamber is agarose gel which is derived from several species of red marine algae, or seaweed. It is also used in size exclusion chromatography. Agarose gel is used to separate and analyze proteins and DNA. In this experiment, agarose gel electrophoresis is used to separate the cut fragments. The DNA fragments are