Yeast Lab6

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Art R. Marina M. Sarah H.!
Mrs. Khaled!
October 12th 2014!

The Effect of Glucose on The Cellular Respiration of Yeast!
Purpose: !
The purpose of this lab is to determine if the quantity glucose solution will increase or decrease the rate at which cellular respiration occurs at within the tested 20% yeast suspension.! Question:!

What are the effects of increasing or decreasing the quantity of 0.06mol/L glucose solution on the cellular respiration within the tested yeast molecules?! Hypothesis: !
When the quantity of glucose solution is increased, the rate at which cellular respiration occurs within the yeast molecules will increase respectively. !
Material:!
-20 mL of 20% yeast suspension ! -20 mL of 0.06 mol/L Glucose solution -Test Tubes ! -0.005% methylene blue solution ! - Test tube holder
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-Beaker
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-Tape! !
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-Goggles
-Hot Plate!!
-Distilled H2O! !
Procedure:!
In this lab, we were observing whether the quantity of glucose solution would effect the rate at which cellular respiration occurs at within the tested yeast. One-by-one, different quantities of glucose solution was added to the corresponding test tubes followed by 6 drops of the 0.005% methylene blue solution. 5 mL of 20% yeast suspension was then added to each test tube . Each tube was placed in the hot water bath and timed to see which reaction would have the greatest alteration on the indicator’s colour (bright blue to clear) within a 5 minute time-frame. The reactions were then rated on a scale of 0-5 (0 being most un-reactive, 5 being most).! 1. 4 test tubes were placed in a test tube holder.!

2. 6 drops of the 0.005% methylene blue solution was added in each test tube.! 3. 3mL of water was added to the control test tube.!
4. 3mL of the glucose solution was added to the first of the four test tubes.! 5. 6mL of the glucose solution was then added to its respective test tube.! 6. 9mL of the glucose solution was added lastly into it’s respective test tube.! 7. Each tube...
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