Trans-Platin Synthesis Lab Report

Though cis-platin is highly effective, its counterpart trans-platin is counterintuitively not (7). Trans-platin is highly reactive with the environments that lead to reaching the DNA, and this reactivity results in the totality of the administered compound becoming deactivated prior to reacting with the DNA (9). Also the DNA lesion caused by cis-platin on the 1,2 intrastrand crosslinks between adjacent purines is an act that trans-platin cannot do when looking from a stereochemical perspective (9). Recently, by adding bulky ligands in replacement of the two amines on trans-platin, antitumor activity has been found, and even activity on cis-platin resistant tumors, suggesting that anticancer mechanism is not limited to lesion causing 1,2 intrastrand
The following paragraph describes the procedures and steps taken on day one of the experiment, which focused entirely on the synthesis of the isomer based on Protocol B. Certain chemical agents used on day one have both health and safety risks, specific details regarding these risks will be explained in more detail in the following procedures, but if unfamiliar with any of these materials it is essential to thoroughly review the safety data sheets of the chemical manufacturers in question. Also all procedures should be done with the use of non-latex laboratory gloves and protective eyewear in a fume hood environment. To a 50ml beaker, 0.268g of potassium tetrachlorplatinate (hazardous), 12.1 ml of deionized water, and a stir bar are added creating a red solution. To this 20 drops from a Pasteur pipette of 2 molar hydrochloric acid (corrosive) is dropped into the beaker. The solution, with the aid of the stir bar above a magnetic hot plate, turns orange. Simultaneously, another hot plate is being heated, upon which the solution is placed, quickly heating it to 88oC. While maintaining solution temperature and vigorously using a stir bar, 6.1ml of 2 molar ammonium hydroxide are added to the solution, turning the orange solution to a very pale yellow. The heating procedure continues at 88oC, being careful as to not overheat the solution past 90oC to avoid unintended
All notes regarding safety presented in the previous paragraph hold true for day two as well. The product created on day one is scraped from the frit and massed using an electronic scale capable of measuring to the thousandth decimal place of the gram. The massing procedure yielded 9 milligrams of product. All of the massed product was then added to a test tube along with a few drops of deionized water, and then the occluded test tube was shaken vigorously in an attempt to dissolve the product. Two other test tubes where also identically prepared with known compounds of cis and transplatin, 21mg and 35mg respectively. All three test tubes where placed in warm mineral oil baths, and a few drops of thiourea (potential carcinogen) where added to each of the test