(Science Investigation Research)
1 YR – ST. LOUIS OF BERTRAND
(Integrated Science Teacher)
Agustin, Nathan Raphael DL.
Arcaira, John Carlo B.
Catuira, Jerrico B.
Olalia, Nicolas Andrei
Serna, Christian O.
Zipagang, Lance Harvey O.
How Caffeine affects a Gold-fish
The Problem and Its Background
* Background of the Study
* Caffeine is becoming a common chemical found in the environment but, little research has been done to understand the environmental effects of caffeine, including dissolved caffeine in aquatic systems. The goal of this research study was to begin to understand how caffeine may interact with aquatic environment, using the bacteria of the genus Pseudomonas, commonly found in the aquatic habitat. We found that Pseudomonas shows an increase in growth when exposed to caffeine, which results in a change in spectrophotometric absorbance, increase in bacteria colony count in the presence of caffeine, and in bio-film like sheen appearing on the glass of the experimental aquarium. Along with the increased bacterial growth, a dramatic increase in ammonia concentration was observed. Ammonia is toxic to fish, and can be correlated with the metabolic activity of the Pseudomonas bacteria, making the caffeinated environment toxic for aquatic life.
* Statement of the Problem
* The statement tells us how those caffeine affects any factors of the fish, on its behavior and its body
* The Hypothesis of the Study
* If caffeine has been presented in an aquatic environment, then the living-things in that surrounding area had a mysterious reaction because caffeine acts us an energy giving nutrient in a human body, making other things more hyper active.
* Significance of the Study
* It makes us know how caffeine affects are body and how much boost it gives us in every beverage we take with it. Coffee contains more than twice the caffeine of most soft drinks and almost twice the caffeine of brewed tea; we can also know how it affects other living-organisms when caffeine is given to them.
* Scope and Delimitation of the Study
* We determine that the temperature has an effect on the respiration rate of the goldfish and, therefore, humans. They state the relationship between rest/exercise and heart/rate or respiration/blood pressure. We predict and observe the effects of different environmental temperatures and caffeine on the respiration rate of a goldfish.
* But, along with the increased bacterial growth, in the tank with caffeine, a dramatic increase in ammonia concentration was observed. Ammonia is toxic to fish, and can be correlated with the metabolic activity of the other bacteria, making the caffeinated environment toxic for aquatic life.
* Definition Terms
* Behavior - refers to the actions and mannerisms made by organisms, systems, or artificial entities in conjunction with its environment, which includes the other systems or organisms around as well as the physical environment.
* Caffeine - is a bitter, white crystalline xanthenes alkaloid that acts as a stimulant drug. Caffeine is found in varying quantities in the seeds, leaves, and fruit of some plants, where it acts as a natural pesticide that paralyzes and kills certain insects feeding on the plants. It is most commonly consumed by humans in infusions extracted from the bean of the coffee plant and the leaves of the tea bush, as well as from various foods and drinks containing products derived from the kola nut. Other sources include yerba maté, guarana berries, guayusa, and theyaupon holly.
* Coffee - is a brewed beverage with a dark, acidic flavor prepared from the roasted seeds of the coffee plant, colloquially called coffee beans. The beans are found in coffee cherries, which grow on trees cultivated in over 70 countries, primarily in equatorial Latin...