Sucrose Metabolic Syndrome Research Paper
Materials and Methods
Animals
The Animal Ethics Committee of the Experimental Toxicology Unit from Medical College of Villa Clara, Cuba, approved experimental protocol. Male and female Wistar rats were used.
All rodents and their food were purchased at the National Center of Laboratory Animal Production (Havana, Cuba).Animals were kept in specific pathogen–free conditions. Room temperature was 22 ± 2 °C on a 12-h:12-h light:dark cycle. Access to food and water was ad libitum.
Sucrose Metabolic Syndrome Induced
Metabolic syndrome was induced from birth to eighteen weeks of life, by drinking a sucrose solution 35% ad libitum, which ensured that all main manifestations of the disease were developed. Three females and one male with sucrose-induced metabolic syndrome were mated. Likewise, we …show more content…
It was used a measuring of blood pressure mark (CODA-UEA) containing sensor to indirectly capture blood pressure values, recording systolic blood pressure (SBP) and diastolic blood pressure (DBP) expressed in mmHg.
External changes and comparing the number, size and fetuses weight
Pregnant rats were undergone to cesarean at 20 days of gestation. This action prevented that the mother rats engulf malformed newborns and stillbirths. Cesarean was performed under ether anesthesia. It was undergone a U-shaped incision in low abdominal region. Then the number of corpora lutea, embryo sacs, early and late resorptions, sexing of the fetus by mother, gravid uterine weight, liver, heart and mother kidneys weight, were registered.
The macroscopic identification of external malformations were carried on after the extraction of the fetuses (Rosario, 2003). Once the external analysis of fetuses completed, evaluations of total number, size and weight of fetuses were made, in order to detect possible differences in the two experimental
Animals
The Animal Ethics Committee of the Experimental Toxicology Unit from Medical College of Villa Clara, Cuba, approved experimental protocol. Male and female Wistar rats were used.
All rodents and their food were purchased at the National Center of Laboratory Animal Production (Havana, Cuba).Animals were kept in specific pathogen–free conditions. Room temperature was 22 ± 2 °C on a 12-h:12-h light:dark cycle. Access to food and water was ad libitum.
Sucrose Metabolic Syndrome Induced
Metabolic syndrome was induced from birth to eighteen weeks of life, by drinking a sucrose solution 35% ad libitum, which ensured that all main manifestations of the disease were developed. Three females and one male with sucrose-induced metabolic syndrome were mated. Likewise, we …show more content…
It was used a measuring of blood pressure mark (CODA-UEA) containing sensor to indirectly capture blood pressure values, recording systolic blood pressure (SBP) and diastolic blood pressure (DBP) expressed in mmHg.
External changes and comparing the number, size and fetuses weight
Pregnant rats were undergone to cesarean at 20 days of gestation. This action prevented that the mother rats engulf malformed newborns and stillbirths. Cesarean was performed under ether anesthesia. It was undergone a U-shaped incision in low abdominal region. Then the number of corpora lutea, embryo sacs, early and late resorptions, sexing of the fetus by mother, gravid uterine weight, liver, heart and mother kidneys weight, were registered.
The macroscopic identification of external malformations were carried on after the extraction of the fetuses (Rosario, 2003). Once the external analysis of fetuses completed, evaluations of total number, size and weight of fetuses were made, in order to detect possible differences in the two experimental