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Streak a Bacterial Culture Anticeptics

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Streak a Bacterial Culture Anticeptics
Introduction
The working hypothesis being tested is if any of the three products; Scope Mouthwash (Scope), LISTERINE® Antiseptic Mouthwash (Listerine), Bactine Original First Aid Liquid (Bactine), and Povidone-Iodine Solution, 10% Topical Microbicide Antiseptic (Iodine) are an appropriate antiseptic to can kill a sample bacterial contaminant found in the local environment, and if so, which is the most effective?
The purpose of this experiment is to determine the antibacterial effectiveness of Scope, Listerine, Bactine, and Iodine in relation to each other.
Scope, Listerine, Bactine, and Iodine each are believed to be effective antiseptics: * Scope Mouthwash - Kills 99% of bad breath germs (in lab tests) http://www.scopemouthwash.com/scope-products/scope-mouthwash-original-mint.aspx * LISTERINE® Antiseptic Mouthwash - This effective rinse kills germs in virtually 100% of the mouth, penetrating to kill plaque biofilm. http://www.jjdentalprofessional.com/deepestclean * Bactine Original First Aid Liquid has provided soothing infection protection for over 50 years. http://www.bactine.com/original.htm * Iodine - A commonly used antimicrobial agent is Povidone-iodine (Betadine), a complex of iodine, the bactericidal component, with polyvinylpyrrolidone (Povidone), a synthetic polymer. http://www.ncbi.nlm.nih.gov/pubmed/9474112

Materials and Methods 1) Step 1 – Collecting and growing a sample bacterial contaminant found in the local environment. a) Materials: i) Sterile cotton swabs. ii) Tube of sterile distilled water. iii) Petri dishes with nutrient agar iv) China Marker v) Object to be tested (all are from the front of the class room): (1) Teachers Chair (2) Computer Keyboard (3) Computer Mouse (4) Teachers Desktop b) Method: vi) draw, with a china marker, on the outside of the agar portion, not the lid, of a Petri



References: Short Section Used to list works of others used in the writing of this report. The purpose of this section is to give credit to those whose work was used to test the hypothesis.

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