Silva Deidra SC121 03 Unit 3 Assignment
LABORATORY REPORT
Activity:
Blood Glucose Regulation
Name:
Deidra Silva
Instructor:
Jeba Inbarasu EdD., PhD
Date:
01.28.2015
Predictions
Plasma glucose levels will be highest immediately after the meal (0 hr)
Plasma ketone levels will be highest
1-3 hours after the meal
Plasma insulin levels will be highest before the meal (fasting)
Plasma glucagon levels will be highest before the meal (fasting)
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
Because you can control the amount of time you spend doing physical activities and the intake amount of both caffeine and alcohol.
5. What type of assay was used to measure plasma glucose and ketone levels? glucose, oxidase, peroxide and dianisidine
6. What type of assay was used to measure plasma insulin and glucagon levels? glucose, oxidase, peroxide and dianisidine
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.2
6.5
6.3
5.7
4.7
Glucose (mmol/l)
Sub. 2
Sub. 3
5.3
5.4
6.4
6.4
6.3
5.7
4.7
6.4
5.6
4.6
average
5.3
6.4
Sub. 1
99
108
6.3
5.7
4.7
59
71
73
Laboratory Report/ Deidra Silva/ Blood Glucose Regulation/ Jeba Inbarasu EdD., PhD/ 01.28.2015/ Page [1] of [4]
Ketones (µmol/l)
Sub. 2
Sub. 3
118
114
114
108
49
77
71
60
57
55
average
110
110
56
68
66
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
Plasma glucose concentration was highest between the time immediately following the meal and one hour after the
Activity:
Blood Glucose Regulation
Name:
Deidra Silva
Instructor:
Jeba Inbarasu EdD., PhD
Date:
01.28.2015
Predictions
Plasma glucose levels will be highest immediately after the meal (0 hr)
Plasma ketone levels will be highest
1-3 hours after the meal
Plasma insulin levels will be highest before the meal (fasting)
Plasma glucagon levels will be highest before the meal (fasting)
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
Because you can control the amount of time you spend doing physical activities and the intake amount of both caffeine and alcohol.
5. What type of assay was used to measure plasma glucose and ketone levels? glucose, oxidase, peroxide and dianisidine
6. What type of assay was used to measure plasma insulin and glucagon levels? glucose, oxidase, peroxide and dianisidine
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.2
6.5
6.3
5.7
4.7
Glucose (mmol/l)
Sub. 2
Sub. 3
5.3
5.4
6.4
6.4
6.3
5.7
4.7
6.4
5.6
4.6
average
5.3
6.4
Sub. 1
99
108
6.3
5.7
4.7
59
71
73
Laboratory Report/ Deidra Silva/ Blood Glucose Regulation/ Jeba Inbarasu EdD., PhD/ 01.28.2015/ Page [1] of [4]
Ketones (µmol/l)
Sub. 2
Sub. 3
118
114
114
108
49
77
71
60
57
55
average
110
110
56
68
66
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
Plasma glucose concentration was highest between the time immediately following the meal and one hour after the