Scientific Paper on Membranes
The Semipermeability of Cell Membrane to Different
Osmotic Environments Using Dialyzing Bag Model1
Sittie Johaynnah M. Sambarani
Group 3 Sec. I-1L
December 17, 2012
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1 A scientific paper in partial fulfillment of the requirements in Biology 10 Laboratory under Professor Junaldo Mantiquilla, 2nd sem., 2012-2
ABSTRACT
The semipermeability of the cell membrane was determined using dialyzing bag models which were exposed into different osmotic conditions. In order to observe the response of animal and plant cells into these conditions, red blood cells (RBCs) and Hydrilla leaf were used and were exposed into different concentrations. Both cells exhibited hypertonic and hypotonic solutions when exposed to high and low concentrations respectively, while exhibiting isotonic when concentrations are equal. Three experiments using dialyzing bags were conducted. The first experiments used three set-ups. The first set-up was a dialyzing bag filled with saturated NaCl solution immersed in distilled water. The second set-up was a dialyzing bag filled with water immersed in saturated NaCl solution, and the last set-up was a dialyzing set-up was dialyzing bag filled with saturated NaCl immersed in saturated NaCl solution. The set-ups were weighed every five minutes for fifty minutes. The next experiment used gelatin granules and distilled water to test if macromolecules could pass through a semipermeable membrane. The change of weight was observed. The last experiment used dialyzing bag filled with NaCl-methylene blue solution. As the Silver nitrate (AgNO3) was dropped, clouds were formed first, proving that salt reacted first than Methylene blue. As observed on the changes of the weight of the dialyzing bags and also on the reactions of plant and animal
Osmotic Environments Using Dialyzing Bag Model1
Sittie Johaynnah M. Sambarani
Group 3 Sec. I-1L
December 17, 2012
-------------------------------------------------
-------------------------------------------------
-------------------------------------------------
-------------------------------------------------
1 A scientific paper in partial fulfillment of the requirements in Biology 10 Laboratory under Professor Junaldo Mantiquilla, 2nd sem., 2012-2
ABSTRACT
The semipermeability of the cell membrane was determined using dialyzing bag models which were exposed into different osmotic conditions. In order to observe the response of animal and plant cells into these conditions, red blood cells (RBCs) and Hydrilla leaf were used and were exposed into different concentrations. Both cells exhibited hypertonic and hypotonic solutions when exposed to high and low concentrations respectively, while exhibiting isotonic when concentrations are equal. Three experiments using dialyzing bags were conducted. The first experiments used three set-ups. The first set-up was a dialyzing bag filled with saturated NaCl solution immersed in distilled water. The second set-up was a dialyzing bag filled with water immersed in saturated NaCl solution, and the last set-up was a dialyzing set-up was dialyzing bag filled with saturated NaCl immersed in saturated NaCl solution. The set-ups were weighed every five minutes for fifty minutes. The next experiment used gelatin granules and distilled water to test if macromolecules could pass through a semipermeable membrane. The change of weight was observed. The last experiment used dialyzing bag filled with NaCl-methylene blue solution. As the Silver nitrate (AgNO3) was dropped, clouds were formed first, proving that salt reacted first than Methylene blue. As observed on the changes of the weight of the dialyzing bags and also on the reactions of plant and animal
Cited: Book Sources: Campbell, N.A and Reece, J.B. 2008. Biology. 8th ed. Singapore: Pearson Education South Asia Pte. Ltd. Pp. 133-134 Chang, R. 2012. Chemistry. 10th ed. New York: McGraw-Hill International Edition p. 534