Porcine Skin

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Characterization of porcine skin as a model for human skin studies using infrared spectroscopic imaging
Rong Kong and Rohit Bhargava*
Downloaded by Queen Mary, University of London on 19/04/2013 18:44:34. Published on 21 April 2011 on http://pubs.rsc.org | doi:10.1039/C1AN15111H

Received 10th February 2011, Accepted 14th March 2011 DOI: 10.1039/c1an15111h Porcine skin is often considered a substitute for human skin based on morphological and functional data, for example, for transdermal drug diffusion studies. A chemical, structural and temporal characterization of porcine skin in comparison to human skin is not available but will likely improve our understanding of this porcine skin model. Here, we employ Fourier transform infrared (FT-IR) spectroscopic imaging to holistically measure chemical species as well as spatial structure as a function of time to characterize porcine skin as a model for human skin. Porcine skin was found to resemble human skin spectroscopically and differences are elucidated. Cryo-prepared fresh porcine skin samples for spectroscopic imaging were found to be stable over time and small variations are observed. Hence, we extended characterization to the use of this model for dynamic processes. In particular, the capacity and stability of this model in transdermal diffusion is examined. The results indicate that porcine skin is likely to be an attractive tool for studying diffusion dynamics of materials in human skin.

Introduction
Skin is a naturally efficient barrier that protects the body from physical and chemical hazards, and the loss of water.1–3 Maintenance of the disease-free function of this vital organ is directly relevant to patients’ health and their social interactions. In particular, skin cancer is the most common form of cancer in the

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