Pompano Fish Lab Report

Fig. 4. Mean (± SEM) weight of pompano fish (g) fed experimental diets on days 0, 14, and 28 of the trial. Treatment diets included the basal diet, or the basal diet with 0.05% Macrogard (D0.05), 0.10% Macrogard (D0.10) and 0.20% Macrogard (D0.20) supplementation. Data are presented as the means ± SEM. Different letters are significantly different between treatments at each time point.

Table 4. Growth performance of pompano fish fed different concentration β-glucan in the diet for 28 days. Data are presented as the means ± SEM. Values in a row with different superscripts differ significantly (P < 0.05).

Diets D0.00 D0.05 D0.10 D0.20
Initial weight (g) 30.53 ± 0.51 30.49 ± 0.62 31.00 ± 0.53 30.83 ± 0.73
Initial length (mm) 101.46
At the end the experiment (day 28), highest biomass yield was in those groups of pompano fish fed D0.05, D0.10 and D0.20, which were significantly higher than fish fed control diet (P ≤ 0.012).

At day 14, HIS of fish did not significantly differ between treatments (P ≥ 0.238), while at day 28 HIS of fish fed D0.05 and D0.10 were significantly higher than HIS of fish fed control (P ≤ 0.040). At days 14, VSI was significantly higher in fish fed D0.05 and D0.10 compared to fish fed control (P ≤ 0.039), but this values did not differ significantly between fish fed D0.20 and fish fed control (P ≥ 0.087). At day 28, all groups of fish fed BG had VSI higher than fish fed control diet (D0.00) (Table 2).

3.7 Survival rate
At the end of the experiment (day 28), survival rates of pompano fish ranged from 86.11% in fish fed D0.00 to 91.66% in fish fed D0.05 (Figure 5). The highest survival was for fish fed diets D0.05 (91.66%) followed by the fish fed D0.10 and D0.20 (90.27% and 89.58%, respectively). The survival rate of fish was not significant difference between the diet treatments (P = 0.083).

Fig. 5. Survival rate of pompano fish fed β-glucan in the diet at days 14 and 28. Data are presented as mean ±
7 and 8). Crude protein content increased significantly with the increasing levels of β-glucan supplementation, and the highest protein content was in fish fed diets D0.05 and D0.10, which was significantly higher than protein content in fish fed D0.00 (P ≤ 0.034). However, there was no significant difference in protein content in fish among the three levels of β-glucan supplementation (P ≥ 0.057). also, no significant difference in protein content in fish fed D0.20 and D0.00 (P = 0.547). Dietary β-glucan significantly reduced lipid content in the muscle of pompano fish in comparison to those fish fed diet D0.00 (P ≤ 0.029), however, lipid contents in fish fed with β-glucan supplementation did not differ significantly from each other (P ≥ 0.221). (Figures 7 and 8)

Fig. 8. Lipid content in the flesh of pompano fish fed different levels of dietary β-glucan

3.10 Correlations between dietary β-glucan and growth, haematological index, microflora and immune response of pompano fish at days 14 and 28.

Table 5. Pearson correlation (r) matrix among dietary β-glucan inclusion (%) and growth performance, haematological index, intestinal microflora and immune response of pompano fish at days 14 and 28. Day 14 Day 28 r Sig. r