Ph Effects On Amylase Enzyme

pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities, one of the enzyme that was used in the process was amylase. When a serial dilution was being prepared, contained starch solution, a buffer, and the amylase enzyme. In results, the absorbance and concentration, decreased as they were added in the other test tubes. The highest concentration occurred at pH 8 and 9 vs. the lowest concentration occurred at pH 5, which had a negative concentration. For most of the pH experiments for tracking enzyme activity over time, concentration decreased as time increased. As the activity was going on during laboratory, investigation of the breakdown of the starch over time was being observed.
2011). The rate of the reaction is the highest at pH of 8 (Reece et al. 2011). According Reece research and laboratory experiment, I agree with the fact that pH 8 is the one when the concentration is highest in the amylase enzyme. If Iodine is added more in the blank one than the concentration of the solution, the results would be inaccurate and most likely to be negative numbers. The enzyme activity stops as soon as the Master Mix touches the iodine solution (Goldsworthy 2015). The null hypothesis is rejected based on the results from the laboratory experiment and Reece’s