Osmosis Lab Report
Abstract The purpose of this experiment was to test different solute concentrations on the rate of osmosis. Artificial cells were filled with different solute concentrations and placed in water and weighed at equal time intervals to show how the water moves across cell membranes and down its concentration gradient into the lower concentrated area. The weights of the cells were recorded each interval, and then the rate of osmosis was found by calculating the corrected cumulative change in weight. The prediction made was that the cells with the higher solute concentrations would have a higher rate of osmosis and the cell filled with water and placed in 40% sucrose solution would have the highest negative weight change.
Introduction
The …show more content…
The experiment begins by softening up the dialysis tubes by soaking them in a beaker of water. When softened, rub the dialysis tubes between your fingers to reveal the opening and clamp the opposite end. Add 10 mL of the appropriate solution to each bag, squeezing the air out of the tube to make sure there are no air bubbles when clamping the other end of the tube. Three of these bags will be filled with 20%, 40% and 60% sucrose solutions. The other two will contain water. Weigh each bag on a balance after clamping and record initial weight. Fill each beaker with the appropriate solution, just enough to cover the bag; four will have water and the fifth beaker will hold 40% sucrose. The bags containing 20%, 40% and 60% will be placed in water, as well as one bag filled with water; the bag of water in water will act as your control. The other bag of water will be placed in the beaker containing 40% sucrose solution. Place the five bags in their rightful beaker simultaneously and record the time. Every ten minutes the bags should be removed, blotted to get the excess solution off and then weighed. Continue weighing the bags every ten minutes for ninety …show more content…
This makes sense because the farther the cell is from osmotic equilibrium, it will gain weight faster (McCutcheon 1926). This supported our prediction. The increase in osmotic rate because of an increase in solute concentration is because water moves from a solution of low solute concentration to a solution with high solute concentration. This means water rushes into a solute of higher concentration faster than a low concentration. This is why the artificial cell with 60% sucrose solution had the highest corrected cumulative change in weight and the fastest osmotic rate. The artificial cell containing water in 40% sucrose solution had a negative osmotic rate because water was leaving the cell and osmosis was occurring in the opposite direction. In this experiment, only one bag of water was placed in 40% sucrose solution. In other experiments, adding two more bags of water and placing them in 20% and 60% sucrose solutions could be tested to see if the rate of osmosis is similar to their 20% and 60% bag counterparts in water. Also, an experiment could be performed to show temperature is a factor in osmotic rates by having the same solute concentration in solutions and placing bags filled with water into them, each having a distinct
Introduction
The …show more content…
The experiment begins by softening up the dialysis tubes by soaking them in a beaker of water. When softened, rub the dialysis tubes between your fingers to reveal the opening and clamp the opposite end. Add 10 mL of the appropriate solution to each bag, squeezing the air out of the tube to make sure there are no air bubbles when clamping the other end of the tube. Three of these bags will be filled with 20%, 40% and 60% sucrose solutions. The other two will contain water. Weigh each bag on a balance after clamping and record initial weight. Fill each beaker with the appropriate solution, just enough to cover the bag; four will have water and the fifth beaker will hold 40% sucrose. The bags containing 20%, 40% and 60% will be placed in water, as well as one bag filled with water; the bag of water in water will act as your control. The other bag of water will be placed in the beaker containing 40% sucrose solution. Place the five bags in their rightful beaker simultaneously and record the time. Every ten minutes the bags should be removed, blotted to get the excess solution off and then weighed. Continue weighing the bags every ten minutes for ninety …show more content…
This makes sense because the farther the cell is from osmotic equilibrium, it will gain weight faster (McCutcheon 1926). This supported our prediction. The increase in osmotic rate because of an increase in solute concentration is because water moves from a solution of low solute concentration to a solution with high solute concentration. This means water rushes into a solute of higher concentration faster than a low concentration. This is why the artificial cell with 60% sucrose solution had the highest corrected cumulative change in weight and the fastest osmotic rate. The artificial cell containing water in 40% sucrose solution had a negative osmotic rate because water was leaving the cell and osmosis was occurring in the opposite direction. In this experiment, only one bag of water was placed in 40% sucrose solution. In other experiments, adding two more bags of water and placing them in 20% and 60% sucrose solutions could be tested to see if the rate of osmosis is similar to their 20% and 60% bag counterparts in water. Also, an experiment could be performed to show temperature is a factor in osmotic rates by having the same solute concentration in solutions and placing bags filled with water into them, each having a distinct