Osmosis Lab

Transport of Solute in Solvent through Osmosis or Diffusion Due to Different Concentration Gradients Passing Through a Semi-permeable Membrane between Cell and Cells Environment
Bio 101

Objective: The objective is to simulate passive transport: diffusion of solutes and osmosis of water through a semipermeable membrane (dialysis tubing). The experiment will show how molecules in solution move from areas of higher concentration to areas of lower concentration in the attempt to reach homeostasis in different circumstances. Introduction: The main purpose of this lab was to observe diffusion and osmosis. This is demonstrated using dialysis tubing and a combination of monosaccharaides, disaccharides, water (H20), and sodium chlorine
Obtain 2mL of each solution from each beaker. This is done using a pipette and pipette. Place the tip of the pipette in the solution of the beaker, and the pipetter is placed at the other end of the pipette. The pippetter sucks the solution into the pipette, this is a better method then using ones mouth to suck up the solution because it is more accurate and safer. Using a hot plate and a beaker filled with water, place each finished test tube into the beaker. The water contained in the beaker must be boiling before each test tube is placed within it. Using this method, the solutions reaction will occur at a much faster rate. This applies to each test. In the (Cl-) test, 2 drops silver nitrate(AgNo3) are added to each 2mL solution that has been measured and placed into a test tube from the obtained material from the cell. This test measures for the salt content in the solution. In the glucose test, Benedict’s test is performed using a 1:1 ratio of Benedict’s test to solution. These tests for the glucose in each solution. Finally the sucrose/lactose test uses the same content as the glucose test. Benedict’s test is performed using a 1:1 ratio of Benedict’s test to solution. This test measures for reducing sugars. The indicator for each test is color. When there is a color change to the solution that means the test has tested
The increase or decrease in mass of the dialysis cell is solely based upon the concentration gradient within the dialysis cell and its environment as to which it is placed in. In each graph, it displays which dialysis cells have increased or decreased in mass. For each cell that has increased in mass, (A1, B1, C1, D1, E1, and F1), diffusion and osmosis has occurred into the cell. As a result the cell has swollen because water has moved into the cell. Dialysis cells that have decreased in mass, (A2, B2, C2, D2, E2, and F2), have had the opposite reaction occur. Diffusion and osmosis has moved water out of the cell, causing a decrease in mass. This is due to the dialysis cell containing a hypotonic solution as its environment at which it was submerged is hypertonic. This then causes the cell to shrink in mass. Due to the different data shown in Graph #1 and Graph #2, we know that the cells and environments contained different concentration gradients because not all data is the same. This means that no dialysis cells or environments have reached equilibrium and have become