Observing Mitosis
Lab Report №3
Title:Observing Mitosis
By Yerkebulan Yesbolatov
Biology course, tutorial group I
October 18, 2012
Lab partner: Azhar Zhaisanova
Introduction
Experiment is intend to show mitotic stage of cell cycle, phases of mitotic stage, to define predominance in the number of each phases over the others, if it is possible. Objectivity of lab is to observe the different phases of mitotic stage of cell cycle, to get skills for special technique( by using orcein ethanoic stain) designed for making slide enable to observe mitotic stage of cell cycle. The tip of Garlic bulb’s root is suspected to be relevant for that, for observing the cell cycle.
Aim
To observe cell cycle in definite stage-in this experiment mitosis.
To get knowledge of which phase is predominate in cell cycle.
Precautions
During the procedure it is need to wear eye protection cause hydrochloric acid is irritant
It is necessary to wear gloves and coat – Toluidine blue is had ability to ingrain. Don’t ingest- it is harmful.
It is necessary to wear goggles and avoiding of content with orsein ethanoic stain, cause it corrosive, irritant, causing burns, has ability to irritate by vapour.
In case of making contact immediately necessary to wash with water for 10 minutes.
It is necessary to wear eye protection and avoid a skin contact, cause Acetic alcohol is corrosive and highly flammable
Methods and Materials
First method of staining is cancelled. Second method with 1-st step was provided in advance. First, several root tips was cut off, added to watch glass with acetic alcohol(2cm3) for 10 minutes. Then, specimens transferred to second glass, which contain 5cm3 ice cold water,4-5 minutes are needed to wait after that. Later on specimens, dried off with filter paper. Next step, in order to haven’t damage a specimen ,it was poured with pre-heated
Title:Observing Mitosis
By Yerkebulan Yesbolatov
Biology course, tutorial group I
October 18, 2012
Lab partner: Azhar Zhaisanova
Introduction
Experiment is intend to show mitotic stage of cell cycle, phases of mitotic stage, to define predominance in the number of each phases over the others, if it is possible. Objectivity of lab is to observe the different phases of mitotic stage of cell cycle, to get skills for special technique( by using orcein ethanoic stain) designed for making slide enable to observe mitotic stage of cell cycle. The tip of Garlic bulb’s root is suspected to be relevant for that, for observing the cell cycle.
Aim
To observe cell cycle in definite stage-in this experiment mitosis.
To get knowledge of which phase is predominate in cell cycle.
Precautions
During the procedure it is need to wear eye protection cause hydrochloric acid is irritant
It is necessary to wear gloves and coat – Toluidine blue is had ability to ingrain. Don’t ingest- it is harmful.
It is necessary to wear goggles and avoiding of content with orsein ethanoic stain, cause it corrosive, irritant, causing burns, has ability to irritate by vapour.
In case of making contact immediately necessary to wash with water for 10 minutes.
It is necessary to wear eye protection and avoid a skin contact, cause Acetic alcohol is corrosive and highly flammable
Methods and Materials
First method of staining is cancelled. Second method with 1-st step was provided in advance. First, several root tips was cut off, added to watch glass with acetic alcohol(2cm3) for 10 minutes. Then, specimens transferred to second glass, which contain 5cm3 ice cold water,4-5 minutes are needed to wait after that. Later on specimens, dried off with filter paper. Next step, in order to haven’t damage a specimen ,it was poured with pre-heated