Laboratory One: Introduction to Microscopy
LABORATORY ONE: INTRODUCTION TO MICROSCOPY
Ang, Elaine
Ceperiano, Arjohn
Dizon, Juan Paulo
Galbraith, Natasha Allen
Lim, Fenina Joyce
Ng, Mary Cathlyn
Ongsiyping, Janelle
Santos, Emmanuel
Ty, Michelle Jenine
Ateneo de Manila University
Loyola Heights Campus Katipunan Avenue, Loyola Heights, Quezon City 1108, Philippines
Key words. Calibration, compound light microscope, magnification, monocot root.
Abstract
This lab activity aims to provide us a comprehensive familiarity with the proper use and handling of the optical microscope, which is a significant instrument in viewing, inspecting and studying the different parts, structures, and components of various botanical specimens.
In this particular experiment, we will study the cross section of a monocot root. In the process, we will become familiar with the parts of the monocot root such as the stele, the central part of the root, and other main internal structures that are found within it such as the metaxylem and protoxylem. (Gifford and Foster 1988)
The main part of the activity is identifying the microscope’s calibration. We will be able to perform such by first determining the ocular micrometer constant from the stage micrometer and ocular micrometer. By determining the ocular micrometer constant, the objective lens constant can be known from the product of the ocular micrometer constant and the eyepiece magnification. The magnification of the illustration can be derived using the monocot root slide. Finally, when these constants are obtained, the magnification of the drawing is obtained.
After calibration and computations, the following findings have been derived:
Ocular micrometer constant 0.01 mm
Objective lens calibration 1 mm
Size of metaxylem 11 ocular units
Magnification:
1.3 cm / 30 mm = 330x magnification
2.5 cm / 50 mm = 550x magnification
I. Introduction
The current trend of technology is that the smaller the product the better; hence, every month companies
Ang, Elaine
Ceperiano, Arjohn
Dizon, Juan Paulo
Galbraith, Natasha Allen
Lim, Fenina Joyce
Ng, Mary Cathlyn
Ongsiyping, Janelle
Santos, Emmanuel
Ty, Michelle Jenine
Ateneo de Manila University
Loyola Heights Campus Katipunan Avenue, Loyola Heights, Quezon City 1108, Philippines
Key words. Calibration, compound light microscope, magnification, monocot root.
Abstract
This lab activity aims to provide us a comprehensive familiarity with the proper use and handling of the optical microscope, which is a significant instrument in viewing, inspecting and studying the different parts, structures, and components of various botanical specimens.
In this particular experiment, we will study the cross section of a monocot root. In the process, we will become familiar with the parts of the monocot root such as the stele, the central part of the root, and other main internal structures that are found within it such as the metaxylem and protoxylem. (Gifford and Foster 1988)
The main part of the activity is identifying the microscope’s calibration. We will be able to perform such by first determining the ocular micrometer constant from the stage micrometer and ocular micrometer. By determining the ocular micrometer constant, the objective lens constant can be known from the product of the ocular micrometer constant and the eyepiece magnification. The magnification of the illustration can be derived using the monocot root slide. Finally, when these constants are obtained, the magnification of the drawing is obtained.
After calibration and computations, the following findings have been derived:
Ocular micrometer constant 0.01 mm
Objective lens calibration 1 mm
Size of metaxylem 11 ocular units
Magnification:
1.3 cm / 30 mm = 330x magnification
2.5 cm / 50 mm = 550x magnification
I. Introduction
The current trend of technology is that the smaller the product the better; hence, every month companies