Lab Report Strawberry Dna
Discussion
Although only the extraction of strawberry DNA was performed in this lab, this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well, and compare it to the steps taken in the strawberry protocol.
As described in the procedure using strawberries, the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective tissue, to have the cells more exposed and to physically break their walls.
Once the strawberry pulp was obtained, the heated salt water detergent was added. As mentioned before, this solution contains water, detergent and salt, and it’s kept warm at 60 degrees Celsius. This mixture plays a major role is dissolving the lipids and proteins that are main constituents of the cell membrane. The latter is known to be made of a bilayer containing phospholipids with hydrophilic heads and hydrophobic tails, in addition to several proteins that are located throughout mosaic fluid. The detergent solution is believed to have the same characteristic and thus, complexes comprised of detergent and the lipids and proteins are formed, which results in the membranes being broken down. The detergent …show more content…
The salty solution comes in play at this level as well to make use of all the positive charges provided by the salts. Those charges stabilize the negative charge of the DNA, and thus allows the molecules to stay attached all together and to separate from their associated proteins (4, 8). The filtration technique is then necessary to purify the DNA sample and have it separated from all the proteins and the other cell organelles. The resulting filtrate contains the DNA as well as some dissolved proteins with the help of the salty
Although only the extraction of strawberry DNA was performed in this lab, this section will address the roles of each step taken and the reagents used during the extraction of DNA from animal tissue as well, and compare it to the steps taken in the strawberry protocol.
As described in the procedure using strawberries, the first step was to mash them into pulp using a mortar and pestle. The main goal from this physical disruption was to break the solid material consisting of any connective tissue, to have the cells more exposed and to physically break their walls.
Once the strawberry pulp was obtained, the heated salt water detergent was added. As mentioned before, this solution contains water, detergent and salt, and it’s kept warm at 60 degrees Celsius. This mixture plays a major role is dissolving the lipids and proteins that are main constituents of the cell membrane. The latter is known to be made of a bilayer containing phospholipids with hydrophilic heads and hydrophobic tails, in addition to several proteins that are located throughout mosaic fluid. The detergent solution is believed to have the same characteristic and thus, complexes comprised of detergent and the lipids and proteins are formed, which results in the membranes being broken down. The detergent …show more content…
The salty solution comes in play at this level as well to make use of all the positive charges provided by the salts. Those charges stabilize the negative charge of the DNA, and thus allows the molecules to stay attached all together and to separate from their associated proteins (4, 8). The filtration technique is then necessary to purify the DNA sample and have it separated from all the proteins and the other cell organelles. The resulting filtrate contains the DNA as well as some dissolved proteins with the help of the salty