Lab 3 Microscopes
BIOLOGY 181 Lab # 4 Microscopy and Cells Lab
A microscope is a tool that enables us to see objects and organisms that are invisible to the naked eye. Microscopes use a series of two or more lenses to magnify an illuminated image. Magnification is a measure of how big an object looks to your eye compared to “life size.” Microscopes also enhance the resolution of an image. Resolution is the ability to distinguish between two objects that are close together. Resolution can be increased using immersion oil. All microscopes have three basic components: 1) magnifying lenses; 2) a means of focusing; and, 3) a source of illumination (light). A compound microscope uses two lenses to view an object. The ocular (eyepiece) is at the top of the microscope …show more content…
Use two hands to transport the microscope. Place one hand under the base at the front of the microscope and the other hand under the handle on the rear of the arm (Figure 2.1).
Figure 4.1 Moving and Handling a Microscope 2. Once the microscope is on the lab bench, connect the power cord to the microscope and plug it into an outlet. 3. Using Figure 2.2 as a guide, locate and identify each ocular and objective lens. Record the magnification of each lens and the total magnification. Total magnification is calculated by multiplying the magnification of the ocular (10x) by the magnification of the objective (10x, 40x, 400x).
Lens Used
10x objective lens 40x objective lens 100x objective lens with oil
Lens Magnification
Total Magnification
4-2
Figure 4.2. Parts of a Microscope 4-3
Figure 4.3. Microscope …show more content…
2. Make a wet mount of your cheek cells (see p. 4-6 for instructions). Examine your slide under the compound microscope and draw a few cheek cells at 400X in the space provided. Be prepared to discuss and present your drawings Make an Elodea leaf wet mount: obtain an Elodea leaf and make a wet mount — do not use methylene blue or other dye in this case. Examine the wet mount with your compound microscope at 400X. Focus on the fleshy layer inside the leaf (do not focus on the surface layer), on the cells filled with chloroplasts (the green bead-like structures). Draw a few of these cells in the space provided; label chloroplasts and cell walls. Do the cells form a tissue? Compare your observation with electron micrograph # 6. Be prepared to discuss and present your drawings Examine “Bacterial Types,” “Columnar Epithelia,” “Human Blood,” “Amoeba,” and “Paramecium.” Examine your slides under the compound microscope and draw cells and tissues in the space provided. Be prepared to discuss and present your drawings after comparing your observations with the following electron micrographs: “Bacterial Types” → “Human Blood” → “Columnar Epithelia” → 4. electron micrograph # 1 electron micrographs # 4 electron micrographs #
A microscope is a tool that enables us to see objects and organisms that are invisible to the naked eye. Microscopes use a series of two or more lenses to magnify an illuminated image. Magnification is a measure of how big an object looks to your eye compared to “life size.” Microscopes also enhance the resolution of an image. Resolution is the ability to distinguish between two objects that are close together. Resolution can be increased using immersion oil. All microscopes have three basic components: 1) magnifying lenses; 2) a means of focusing; and, 3) a source of illumination (light). A compound microscope uses two lenses to view an object. The ocular (eyepiece) is at the top of the microscope …show more content…
Use two hands to transport the microscope. Place one hand under the base at the front of the microscope and the other hand under the handle on the rear of the arm (Figure 2.1).
Figure 4.1 Moving and Handling a Microscope 2. Once the microscope is on the lab bench, connect the power cord to the microscope and plug it into an outlet. 3. Using Figure 2.2 as a guide, locate and identify each ocular and objective lens. Record the magnification of each lens and the total magnification. Total magnification is calculated by multiplying the magnification of the ocular (10x) by the magnification of the objective (10x, 40x, 400x).
Lens Used
10x objective lens 40x objective lens 100x objective lens with oil
Lens Magnification
Total Magnification
4-2
Figure 4.2. Parts of a Microscope 4-3
Figure 4.3. Microscope …show more content…
2. Make a wet mount of your cheek cells (see p. 4-6 for instructions). Examine your slide under the compound microscope and draw a few cheek cells at 400X in the space provided. Be prepared to discuss and present your drawings Make an Elodea leaf wet mount: obtain an Elodea leaf and make a wet mount — do not use methylene blue or other dye in this case. Examine the wet mount with your compound microscope at 400X. Focus on the fleshy layer inside the leaf (do not focus on the surface layer), on the cells filled with chloroplasts (the green bead-like structures). Draw a few of these cells in the space provided; label chloroplasts and cell walls. Do the cells form a tissue? Compare your observation with electron micrograph # 6. Be prepared to discuss and present your drawings Examine “Bacterial Types,” “Columnar Epithelia,” “Human Blood,” “Amoeba,” and “Paramecium.” Examine your slides under the compound microscope and draw cells and tissues in the space provided. Be prepared to discuss and present your drawings after comparing your observations with the following electron micrographs: “Bacterial Types” → “Human Blood” → “Columnar Epithelia” → 4. electron micrograph # 1 electron micrographs # 4 electron micrographs #