Hydrogen Peroxide Experiment Lab Report
Catalase Experiment
Research Question: How does the weight of a piece of liver (0.4g, 0.8g, 1.2g and 1.6g in this case) affect the amount of gas created after 2 minutes when combined with hydrogen peroxide?
Aim: To see find out how the amount of catalase correlates with the amount of gas created when in combination with hydrogen peroxide.
Materials:
15g of liver
25 ml of hydrogen peroxide
1 scale
1 bucket
1 100ml graduated cylinder
1 250ml flask with bung
1 50cm tube (able to fix onto top of flask)
1 stopwatch
1 100ml beaker
1 funnel
1 pipette
1 microscopy kit
Variables:
Independent: This is the amount of liver added to the hydrogen peroxide. The initial weight will be 0.4 grams will increase by 0.4g up until
Control: The amount of hydrogen peroxide will be kept the same. 3ml will be added each test. The temperature will be mainly set at room temperature (approximately 20⁰-25⁰). The pH of the hydrogen peroxide will also remain the same. …show more content…
Set-up:
Method:
1. Set up the equipment as shown above.
2. Fill the bucket and graduated cylinder with water (so that there is no air in the cylinder)
3. Cut and weigh 3 pieces of liver weighing 0.4 g each using the microscopy kit and scale.
4. Place one piece of liver in the 250 ml flask and then add 3ml of hydrogen peroxide.
5. Immediately put the bung in the flask and start the stopwatch.
6. Swirl the flask around lightly making sure the hydrogen peroxide and liver are constantly in contact with each other.
7. Stop the stopwatch after 2 minutes and check how much water was pushed out of the graduated cylinder by the gas.
8. Record this result and repeat another 2 times.
9. Then repeat this 9 more times but with weights of 0.8g, 1.2g and
Research Question: How does the weight of a piece of liver (0.4g, 0.8g, 1.2g and 1.6g in this case) affect the amount of gas created after 2 minutes when combined with hydrogen peroxide?
Aim: To see find out how the amount of catalase correlates with the amount of gas created when in combination with hydrogen peroxide.
Materials:
15g of liver
25 ml of hydrogen peroxide
1 scale
1 bucket
1 100ml graduated cylinder
1 250ml flask with bung
1 50cm tube (able to fix onto top of flask)
1 stopwatch
1 100ml beaker
1 funnel
1 pipette
1 microscopy kit
Variables:
Independent: This is the amount of liver added to the hydrogen peroxide. The initial weight will be 0.4 grams will increase by 0.4g up until
Control: The amount of hydrogen peroxide will be kept the same. 3ml will be added each test. The temperature will be mainly set at room temperature (approximately 20⁰-25⁰). The pH of the hydrogen peroxide will also remain the same. …show more content…
Set-up:
Method:
1. Set up the equipment as shown above.
2. Fill the bucket and graduated cylinder with water (so that there is no air in the cylinder)
3. Cut and weigh 3 pieces of liver weighing 0.4 g each using the microscopy kit and scale.
4. Place one piece of liver in the 250 ml flask and then add 3ml of hydrogen peroxide.
5. Immediately put the bung in the flask and start the stopwatch.
6. Swirl the flask around lightly making sure the hydrogen peroxide and liver are constantly in contact with each other.
7. Stop the stopwatch after 2 minutes and check how much water was pushed out of the graduated cylinder by the gas.
8. Record this result and repeat another 2 times.
9. Then repeat this 9 more times but with weights of 0.8g, 1.2g and