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Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato

Aim: To investigate the Effect of temperature (10, 37, 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start a reaction.Enzymes are affected by several factors including PH, Substrate concentration; Temperature & other factors. Each enzyme has an optimum temperature at which its activity is the highest, below this optimum temp, the kinetic energy of molecules decrease , therefore the collisions between the active site of the enzyme and substrate decreases , as a result the enzyme activity will decrease , so decreasing the rate of the reaction If the temp. Exceeds the optimum temp. The kinetic energy between molecules increase therefore collisions increase leading to the change in the tertiary structure of the enzyme and in this case active site is lost and the enzymes will be denatured so the reaction will slow down &stops. Catalase is an enzyme, found basically in all living cells. It breaks down hydrogen peroxide (waste product) into water and oxygen.

2H₂O₂ 2H2O+O2
As predicted, the enzyme catalase activity would be the highest at 37c ͦ (Optimum temp.)if increased to 60c ͦ then the enzyme would be denatured and if decreased to 10c ͦ (very low temp.) then the reaction would be slow. Variables:

Dependent: Height of oxygen bubbles (cm) using a ruler.
Independent: Temperature (10c ͦ, 37c ͦ, 60c ͦ) using three different water baths each adjusted to a specific temp . Controlled:
1. Number of potato cubes: 3 cubes of potatoes were used in each trial at each different temp. If changed, whether decrease or increase, then the number of enzymes...

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