Gel Electrophoresis Research Paper
Restriction Enzymes and Electrophoresis
Bhumik Patel
Phillips
1/16/11
Restriction enzymes are tools in DNA research that can cut DNA into exactly needed pieces. Certain cuts can be rough, while others can be clean. Certain cuts can have an organized pattern to have a staggered cut. Other cuts will leave complementary bases with them. Electrophoresis allows the manipulation of DNA to separate and organize those parts. Electrophoresis is the substrate electric movement of the separation of DNA. Gel Electrophoresis can separate both DNA and RNA. This can also allow the knowledge of the unknown bases of a strand of DNA. This can allow the knowledge of familiar patterns that can create a unique signature. Separation of these molecules …show more content…
Compare the banding patterns formed in each lane of the gel. Assume it is the same as the banding pattern on an autoradiograph. Do you think the three DNA samples tested are the same? Explain. How can you further verify weather or not any of the DNA samples tested are the same?
A: No, because the samples would lead to, if they are all the same, that all of the suspects have committed the crime or that they are identical twins. You could have done a lie detector test as a further verifier. 2. Which of the two suspects if the real burglar? Explain you answer.
A: The second suspect is more likely to be the burglar because the DNA matches more on the second suspect than the first one. 3. Assume you have to identify two unknown DNA samples. Explain the function of each step in the DNA fingerprinting technique. a) Restriction Enzyme Digestion cleaves and cuts DNA in certain spots in to fragments. b) Gel Electrophoresis can be used to separate and organize the DNA fragments. c) Denaturation into Single-Stranded DNA this will divide those fragments in to more distinct categories and fragment
Bhumik Patel
Phillips
1/16/11
Restriction enzymes are tools in DNA research that can cut DNA into exactly needed pieces. Certain cuts can be rough, while others can be clean. Certain cuts can have an organized pattern to have a staggered cut. Other cuts will leave complementary bases with them. Electrophoresis allows the manipulation of DNA to separate and organize those parts. Electrophoresis is the substrate electric movement of the separation of DNA. Gel Electrophoresis can separate both DNA and RNA. This can also allow the knowledge of the unknown bases of a strand of DNA. This can allow the knowledge of familiar patterns that can create a unique signature. Separation of these molecules …show more content…
Compare the banding patterns formed in each lane of the gel. Assume it is the same as the banding pattern on an autoradiograph. Do you think the three DNA samples tested are the same? Explain. How can you further verify weather or not any of the DNA samples tested are the same?
A: No, because the samples would lead to, if they are all the same, that all of the suspects have committed the crime or that they are identical twins. You could have done a lie detector test as a further verifier. 2. Which of the two suspects if the real burglar? Explain you answer.
A: The second suspect is more likely to be the burglar because the DNA matches more on the second suspect than the first one. 3. Assume you have to identify two unknown DNA samples. Explain the function of each step in the DNA fingerprinting technique. a) Restriction Enzyme Digestion cleaves and cuts DNA in certain spots in to fragments. b) Gel Electrophoresis can be used to separate and organize the DNA fragments. c) Denaturation into Single-Stranded DNA this will divide those fragments in to more distinct categories and fragment