Enzyme Catalase Lab Report
Activity of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity
Introduction
The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2), which is a toxic chemical to the human body. When hydrogen peroxide is broken down, the end products are Water (H2O) and Oxygen (O2). In this report, the reaction of catalase to hydrogen peroxide is being tested. Furthermore, the effects of temperature, concentration, and pH level on this reaction are being considered.
We hypothesize that he experiments will show that Catalase speeds the breakdown of hydrogen peroxide to water and oxygen, and the oxygen will produce a larger bubbling effect in the …show more content…
Catalase was added to the 1 cm mark of test tube 1 and test tube 3, while test tube 2 was filled to the 1 cm mark with water. Hydrogen peroxide was added to the 5 cm mark in test tube 1 and test tube 2, while a sucrose solution was used to fill test tube 3. All test tubes were mixed and we waited 20 seconds for bubbling. Test tube 1 represented the experiment, while test tube 2 was the negative control for hydrogen peroxide, and test tube 3 was the negative control for the …show more content…
The concentration of catalase and hydrogen peroxide are again the same as in test tube one in the first experiment. However, 2 cm of water adjusted to different pH levels were added. In test tube 1, 2 cm of water with pH of 3 is added to the 1 cm of catalase and 4 cm of hydrogen peroxide. In test tube 2, 2 cm of water adjusted to the pH adjusted to 7 was added to 1 cm of catalase and 4 cm of hydrogen peroxide. In test tube 3, 2 cm of water adjusted to the pH adjusted to 11 was added to 1 cm of catalase and 4 cm of hydrogen
Introduction
The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2), which is a toxic chemical to the human body. When hydrogen peroxide is broken down, the end products are Water (H2O) and Oxygen (O2). In this report, the reaction of catalase to hydrogen peroxide is being tested. Furthermore, the effects of temperature, concentration, and pH level on this reaction are being considered.
We hypothesize that he experiments will show that Catalase speeds the breakdown of hydrogen peroxide to water and oxygen, and the oxygen will produce a larger bubbling effect in the …show more content…
Catalase was added to the 1 cm mark of test tube 1 and test tube 3, while test tube 2 was filled to the 1 cm mark with water. Hydrogen peroxide was added to the 5 cm mark in test tube 1 and test tube 2, while a sucrose solution was used to fill test tube 3. All test tubes were mixed and we waited 20 seconds for bubbling. Test tube 1 represented the experiment, while test tube 2 was the negative control for hydrogen peroxide, and test tube 3 was the negative control for the …show more content…
The concentration of catalase and hydrogen peroxide are again the same as in test tube one in the first experiment. However, 2 cm of water adjusted to different pH levels were added. In test tube 1, 2 cm of water with pH of 3 is added to the 1 cm of catalase and 4 cm of hydrogen peroxide. In test tube 2, 2 cm of water adjusted to the pH adjusted to 7 was added to 1 cm of catalase and 4 cm of hydrogen peroxide. In test tube 3, 2 cm of water adjusted to the pH adjusted to 11 was added to 1 cm of catalase and 4 cm of hydrogen