Effect of Iron Oxide and Gold Nanoparticles on Bacterial Growth Leading Towards Biological Application

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Journal of Nanobiotechnology
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Effect of iron oxide and gold nanoparticles on bacterial growth leading towards biological application Journal of Nanobiotechnology 2011, 9:34 doi:10.1186/1477-3155-9-34

Saptarshi Chatterjee (saptarshi_gcc07@yahoo.co.in) Arghya Bandyopadhyay (arghya.micro@gmail.com) Keka Sarkar (keka@klyuniv.ac.in)

ISSN Article type Submission date Acceptance date Publication date Article URL

1477-3155 Research 30 June 2011 23 August 2011 23 August 2011 http://www.jnanobiotechnology.com/content/9/1/34

This peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below). Articles in JN are listed in PubMed and archived at PubMed Central. For information about publishing your research in JN or any BioMed Central journal, go to http://www.jnanobiotechnology.com/authors/instructions/ For information about other BioMed Central publications go to http://www.biomedcentral.com/

© 2011 Chatterjee et al. ; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

EFFECT OF IRON OXIDE AND GOLD NANOPARTICLES ON BACTERIAL GROWTH LEADING TOWARDS BIOLOGICAL APPLICATION

Saptarshi Chatterjee, Arghya Bandyopadhyay, Keka Sarkar Department of Microbiology, University of Kalyani Nadia, West Bengal, India

SC: saptarshi_gcc07@yahoo.co.in AB: arghya.micro@gmail.com KS: keka@klyuniv.ac.in

ABSTRACT: Background: Nanoparticle- metal oxide and gold represents a new class of important materials that are increasingly being developed for use in research and health related activities. The biological system being extremely critical requires the fundamental understanding on the influence of inorganic nanoparticles on cellular growth and functions. Our study was aimed to find out the effect of iron oxide (Fe3O4), gold (Au) nanoparticles on cellular growth of Escherichia coli (E. coli) and also try to channelize the obtained result by functionalizing the Au nanoparticle for further biological applications. Result: Fe3O4 and Au nanoparticles were prepared and characterized using Transmission electron microscopy (TEM) and Dynamic Light Scattering (DLS). Preliminary growth analysis data suggest that the nanoparticles of iron oxide have an inhibitory effect on E. coli in a concentration dependant manner, whereas the gold nanoparticle directly showed no such activity. However the phase contrast microscopic study clearly demonstrated that the effect of both Fe3O4 and Au nanoparticle extended up to the level of cell division which was evident as the abrupt increase in bacterial cell length. The incorporation of gold nanoparticle by bacterial cell was also observed during microscopic analysis based on which glutathione functionalized gold nanoparticle was prepared and used as a vector for plasmid DNA transport within bacterial cell. Conclusion: Altogether the study suggests that there is metal nanoparticle - bacteria interaction at the cellular level that can be utilized for beneficial biological application but significantly it also posses potential to produce ecotoxicity, challenging the ecofriendly nature of nanoparticles.

Key Words: Bacterial Growth, magnetic nanoparticle, gold nanoparticle, Cytotoxicity.

BACKGROUND: The present era belongs to nanotechnology. With the tremendous growth in the field of science, nanobiotechnology has come up as a major interdisciplinary subject. The development and application of nanotechnology has the potential to improve greatly the quality of life. An improved understanding of...
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