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dependent/independent light lab

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dependent/independent light lab
Light Lab
Introduction:
The purpose of this lab is to observe how influential light is to the construction and/or use of CO2 and O2 amongst plants. This can be examined by experimenting the effects of plants under both light and no light. In order to begin this experiment you must begin by wrapping the “BioChamber” with aluminum foil to prevent light from coming through to the leaves, however, once the lid has been covered you are to poke holes so you can insert the sensors. Next, you will cover the bottom of the “BioChamber” with one layer of turgid spinach leaves and proceed by connecting the Gas Sensor to Channel 1 and the O2 Gas to Channel 2 of the “Vernier computer interface”. Once the file “31C Photo (CO2 and O2)” is opened you can secure the lid on the chamber and insert the sensors into the holes that you previously made. After 15-minutes of data being collected you can observe the data. Now that you have completed the light independent reaction, you can begin by setting up the light dependent reaction. First, assemble the lamp without turning it on until told to do so. Remove the aluminum foil and align the bottom of the chamber with turgid spinach leaves. Re-secure the lid on the chamber and connect the sensors. Place the chamber inside the bulb and turn on the lamp. You will then reopen the selected file and review 15-minutes of collected data. Furthermore, when reading over the procedure you can conclude that the controls of this experiment are the layers of turgid spinach layers, the container, the sensors, and the aluminum foil. The variable of this experiment, nonetheless, is the amount of light used throughout the experiment. The reason for this is because you begin with no light and then use the light for the second set of data. Given that we are testing the amount of CO2 and O2 produced by spinach leaves depending on the amount of light causes opposite results. If the chamber were placed under a turned on bulb, there would be a

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