Deltamethrin Lab Report

Biodegradation of Deltamethrin from S1 Fungal Isolate
Janice Dsouza, Surajvanshikumar Suvarna, Seekha Parida, Anudurga Gajendiran and Jayanthi Abraham
VIT UNIVERSITY
VELLORE
Abstract: Microbial biodegradation is a promising approach for recovery of environmental sites contaminated with pesticides and an effective way to remove toxicants. Deltamethrin is the most commonly used pyrethroid in agricultural practices commonly used in different geographical parts of the world. It is detected in many environmental areas such as soil and water, posing a toxic effect on humans and other organisms. In the current study, a fungal S1 strain was isolated and enriched from soil sample originally obtained from Vellore agricultural site. The 18s rRNA of the
A series of 250ml Erlenmeyer flasks containing 100ml of M1 medium (0.2g NaNO3, 0.05g KCl, 0.05g MgSO4.7H2O, 1g glucose, 0.001g FeCl3, 0.02 g BaCl2, 0.05g CaCl2) at pH 6.8 with an addition of increasing concentration of deltamethrin was prepared. 1ml of 108 fungal spore suspension cultures was added into each of these flasks. These flasks were incubated at 280± 20C on the shaker at 120rpm for 7 days. Mycelium growth was observed in each of these flasks. The mycelium growth was measured by filtering the contents using Whatman Filter paper No.1 from each of the flasks. The filter paper was washed with deionized water and then dried at 750C in a hot air oven till completely dried and was weighed in a pre-weighed Petri dish to obtain the residual fungal mass. The lowest concentration at which the fungal mycelium growth inhibited was chosen as the MIC of deltamethrin (Abraham and Silambarasan,
1ml of logarithmic phase (108 spores/ml) fungal spore suspension was added into each of these flasks. These flasks were incubated at 280± 20C on the shaker at 120rpm for 5 days. One from each set of these flasks were removed and measured for fungal dry weight at 24,48,72,96 and 120hrs. The fungal dry weight obtained determined the growth pattern of the isolate in the aqueous medium.
Biodegradation studies of deltamethrin in aqueous medium and determination of its metabolites.
To determine the degradation of deltamethrin in aqueous medium, 1 milliliter of 108 fungal spore suspension was dispensed into 250ml Erlenmeyer flasks containing 100ml of modified M1 medium with deltamethrin as the sole source of carbon. This flask was incubated at 280± 20C on the shaker at 120rpm. 7ml of the culture sample is withdrawn aseptically at regular day intervals for the determination of pesticide concentration and its metabolites through HPLC analysis. (Logeswari et al.,