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Chromatography Of Unknown Samples And Paprik Analysis Of Acetaminophen

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Chromatography Of Unknown Samples And Paprik Analysis Of Acetaminophen
Thin Layer Chromatography of Unknown Samples and Paprika
By: Uyen Huynh
Date: June 18, 2014
Lab Partners: Philip Murray
Professor and section: M. Pandey, CHM2210L.601

Data:
Analysis of Drug Store Items
Knowns
Rf Values
Aspirin
.91 cm
Acetaminophen
.85 cm
Ascorbic Acid
.86 cm
Unknown
.97 cm
Identity of unknown mixture
Acetaminophen

Analysis of Paprika Pigments
Spot Number
Rf
Color
1
1
Red-orange
2
1
Red-orange

Calculations A. Analysis of Paprika picture sample
B. Flower sample for paper chromatography
Analysis of Drug Store Items
Knowns
Rf Values= a/f
Aspirin
7.0/7.7= .91 cm
Acetaminophen
7.7/7.7= 1 cm
Ascorbic Acid
6.6/7.7= .86 cm
Unknown
7.5/7.7= …show more content…
We found the Rf value by the distance spot travels divided by the distance the solvent travels. From the TLC samples collected, the solvent travels to be 7.7 cm and the distance the spot travels from aspirin, acetaminophen, ascorbic acid, and unknown to be 7.0 cm, 7.7 cm, 7.6 cm, and 7.5 cm respectively. Since the two …show more content…
Our experiment proved our hypothesis to be somewhat right because we did see most of it without the UV light but there were some parts on towards the middle that we could only see with the UV light which means about half of it was colorless and couldn’t be seen under regular lighting. For the paprika experiment since it had color, we could see all of the spots on the plate under regular lighting and we did try putting it under UV light as it was the same spots seen under regular lighting so we concluded that whatever was colorless from the beginning couldn’t be seen in regular lighting and whatever sample had color could be seen under regular
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