Chloramphenicol: The Effects Of Bacterial Microorganisms
There were eight antibiotics used for this experiment; chloramphenicol (C), erythromycin (E), fusidic acid (FC), oxacillin (OX), novobiocin (NO), penicillin G (PG), streptomycin (S), and tetracycline (T).
The mode of action of Chloramphenicol is the inhibition of protein synthesis. Chloramphenicol irreversibly binds to a receptor sit on the 50S subunit of the ribosome on the bacteria that inhibits the enzyme peptidyl transferase. This inhibition results to the prevention of the transfer of amino acid for growing peptide chains which leads to the inhibition of the formation of bacterial protein. (Michigan State University, 2011). Chloramphenicol is a broad-spectrum antibiotic. (Hitner et al., 2016). Bacterial microorganisms become resistant by the inactivation of chloramphenicol through the enzyme acetyltransferases. (Rang et al., 2016). Chloramphenicol resistance are very common in Staphylococcus spp. such as Staphylococcus aureus (S. aureus) and Staphylococcus epidermis (S. epidermis). The enzyme that inactivates chloramphenicol appears to be the product of a structural gene on the chloramphenicol plasmid due to the resistance and enzyme activity. (Shaw et al., 1970) The inactivation enzyme was produced by the resistant strains of both Gram-positive and Gram-negative organisms. In Gram-negative bacteria, the enzyme is generated …show more content…
It is stable against penicillinase. It is used to treat many different types of infections caused by bacteria such as penicillinase-causing staphylococcal infection. This includes bacteraemia, skin and soft-tissue infections, respiratory tract infections, bone and joint infections, and urinary tract infections. (Prescribers’ Digital Reference, 2017) Oxacillin cannot be used on individuals that have allergies towards other penicillin antibiotics. Oxacillin must be taken on an empty stomach, at least 1 hour before or 2 hours after eating a meal. (Cerner Multum, Inc.,
The mode of action of Chloramphenicol is the inhibition of protein synthesis. Chloramphenicol irreversibly binds to a receptor sit on the 50S subunit of the ribosome on the bacteria that inhibits the enzyme peptidyl transferase. This inhibition results to the prevention of the transfer of amino acid for growing peptide chains which leads to the inhibition of the formation of bacterial protein. (Michigan State University, 2011). Chloramphenicol is a broad-spectrum antibiotic. (Hitner et al., 2016). Bacterial microorganisms become resistant by the inactivation of chloramphenicol through the enzyme acetyltransferases. (Rang et al., 2016). Chloramphenicol resistance are very common in Staphylococcus spp. such as Staphylococcus aureus (S. aureus) and Staphylococcus epidermis (S. epidermis). The enzyme that inactivates chloramphenicol appears to be the product of a structural gene on the chloramphenicol plasmid due to the resistance and enzyme activity. (Shaw et al., 1970) The inactivation enzyme was produced by the resistant strains of both Gram-positive and Gram-negative organisms. In Gram-negative bacteria, the enzyme is generated …show more content…
It is stable against penicillinase. It is used to treat many different types of infections caused by bacteria such as penicillinase-causing staphylococcal infection. This includes bacteraemia, skin and soft-tissue infections, respiratory tract infections, bone and joint infections, and urinary tract infections. (Prescribers’ Digital Reference, 2017) Oxacillin cannot be used on individuals that have allergies towards other penicillin antibiotics. Oxacillin must be taken on an empty stomach, at least 1 hour before or 2 hours after eating a meal. (Cerner Multum, Inc.,