CHEM 2303 Lecture 2 Jan 16 2015 A

CHEM 2303

ANALYTICAL CHEMISTRY II
LECTURE 2 – APPLICATIONS OF SPECTROPHOTOMETRY

TENTATIVE LECTURE SCHEDULE
Date

Topics
• Presentation / discussion of syllabus
• Tips for success
• Laboratory
• Introduction to the analytical process
• Sample preparation
Jan 9th, 2015
• Spectrophotometry
• Properties of light
• Absorption of light
• Excited states
• Luminescence
• Applications of spectrophotometry
• UV-Vis spectroscopy
• Fluorimetry
Jan 16th, 2015
• Fluorescent staining, green fluorescent protein
• Immmunoassays
• Discussion on lab test
• Optional tutorial on Ch. 1, 2, 3, 4-1 and 4-2 (8:359:20am)
Jan 23rd, 2015 - LAB
• Error calculations (not on lab test)
TEST
• 10 minute break
• Test on Ch 1, 2, 3, 4-1 and 4-2 (9:30-11:25am)

- Lecture and test / Midterm schedule is subject to change at the discretion of Instructor

Chapters (N = Notes)
Lecture Slides
Lecture Slides
See lab maunal
Ch. 0
Ch. 27-2
Ch. 17
Ch. 17-1
Ch. 17-2
Ch. 17-6
Ch. 17-7
Ch. 18, 19
Ch. 19-1,-2,-3, N
Ch. 17-7, N
N
Ch. 18-5, N
Ch. 1, 2, 3, 4-1, 4-2
Ch. 1, 2, 3, 4-1, 4-2
Ch. 4-8
N/A
Ch. 1, 2, 3, 4-1 and 4-2

UV-Vis spectroscopy – Ch. 17-3

A = εbc = -logT

T=

??
??0

http://www.cofc.edu/~kinard/221LCHEM/CHEM221L%20Spectrophotometric%20Determination%20of%20Iron.htm

A single beam instrument
P0 (or I0 above) is not measured directly – must use a
“blank”

UV-Vis spectroscopy – Ch. 17-3

Figure 17-5
Cuvets must be kept clean!!

UV-Vis spectroscopy – Ch. 19
Double beam scanning spectrophotometer

Figure 19-1

Again, cuvets must be kept clean!!
Cuvets must be identical to each other.

UV-Vis spectroscopy – Ch. 19

UV-Vis spectroscopy – Ch. 19
Deuterium arc lamp Disperses light into various λ
Quartzhalogen (like a headlight on a car) Again, cuvets must be kept clean and must be identical to each other.

Selects narrow band of λ
Figure 19-2

Sources of light – Ch. 19-1
Tungsten lamp:
• excellent source of visible and near-IR radiation • operates at temperature near
3000 K
• produces useful