Cellular Respiration Lab Report

LAB FIVE CELL RESPIRATION
INTRODUCTION
Aerobic cellular respiration is the release of energy from organic compound from organic compounds by metabolic chemical oxidation in the mitochondria within each cell. Cellular respiration involves a series of enzyme-mediated reactions. The equation below shows the complete oxidation of glucose. Oxygen is required for this energy-releasing process to occur. C6H12O6 + 6O2 -> 6CO2 + 6H2O + 686 kilocalories of energy/mole of glucose oxidized
PURPOSE
Cellular respiration is an ATP-producing catabolic process in which the electron receiver is an inorganic molecule. It is the release of energy from organic compounds by chemical oxidation in the mitochondria within each cell. Carbohydrates, proteins, and fats can all be metabolized, but cellular respiration usually involves glucose: C6H12O6 + 6O2 → 6CO2 + 6H2O + 686 Kcal of energy/mole of glucose oxidized. Cellular respiration involves glycolysis, the Krebs cycle, and the electron transport chain. Glycolysis is a catabolic pathway that occurs in the cytosol and partially oxidizes glucose into two pyruvate (3-C). The Krebs cycle occurs in the mitochondria and breaks down a pyruvate (Acetyl-CoA) into carbon dioxide. These two cycles both produce a small amount of ATP by substrate-level phosphorylation and NADH by transferring electrons from substrate to NAD+. The Krebs cycle also produces FADH2 by transferring electrons to FAD. The electron transport chain is located at the inner membrane of the mitochondria and accepts energized electrons from enzymes that are collected during glycolysis and the Krebs cycle, and
Two hypothesis. Germinating peas should consume more oxygen than non-germinating peas. Peas germinating at warm temperatures should consume more oxygen than peas germinating at cold temperatures
2.This activity uses a number of controls. What conditions must be kept constant?.
Water baths held at constant temperature
Volume of KOH is the equal in every tube
Equilibration time is identical for all respirometers.
3.Describe and explain the relationship between the amount of oxygen consumed and time? The amount of oxygen consumed was greatest in germinating peas in warm water. The oxygen consumption increased over time in germinating peas.
4.

Condition | Show Calculations | Rate in mL O / minute | Germinating peas at 10oC | 2.3-1.5=.8/5 | .16mL O2 /minute | Germinating peas at room temperature | 4.6-3.1/5 | .3mL O2 /minute | Dry peas at 10oC | (.1)/5= | .02 mL O2 /minute | Dry peas at room temperature | (.2-0 )/5= | .04 mL O 2 /minute