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Calcium Chloride Lab Report

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Calcium Chloride Lab Report
A transformation solution of Calcium Chloride was obtained and then separated into two test tubes of 250μl portions and placed on ice. A sample of the bacterium Escherichia coli was then immersed and mixed into each of these tubes. Then a sample of pGLO plasmid DNA containing GFP was acquired and placed into only one of the previous test tubes, and both tubes were incubated on ice for ten minutes. 4 agar plates of different composition were prepared. Both tubes were then put through a 420C heat shock for 50 sec, then the tubes were immediately placed back on ice and incubated for another 2 min. A 250μl of LB nutrient broth was then added and mixed into each tube. 100μl of the transformation containing both the plasmid and the bacterium was
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