C311
Experiment 1: Pipet Calibration and Excel C311: Section 8919 5/18/2009
Purpose:
The purpose of the pipet calibration is to determine the accuracy and precision of 10ml of water at room temperature by using a 10ml volumetric pipet. Also, analyzing the analytical balance and the density of water from literature reference.
Experimental:
Description of glassware, equipments, and materials:
10 ml volumetric pipet (+0.02ml) Computer program: Excel oven
(1) 50 ml beaker Thermometer desiccator
(1) 250 ml beaker #1 stopper DI water
(1) 400 ml beaker 50 ml Erlenmeyer flask
Analytical balance (+0.0001g)
A clean 250 ml beaker was filled with 200ml of DI water and put aside to equilibrate to room temperature. Temperature was taken with a thermometer. A 50 ml Erlenmeyer flask was then cleaned rinsed and removed from water. The flask was marked with a pen and placed in oven to dry, then placed in desiccator to cool. The 10 ml volumetric pipet was cleaned with hot soapy water and rinsed with DI water before use. The mass of the empty flask with stopper was measured by analytical balance. A transfer quickly of 10 ml aliquot of DI water to flask replacing stopper; the mass was taken. The procedure was repeated three more times by adding additional 10 ml of water to flask and obtaining the mass after each addition.
Data Tables and Equations:
Summary Table 1: Mass and Volume of Water at 24⁰C
#1 #2 #3 #4 mi of flask (g)
48.1267
58.0784
68.0556
78.0387 mf of flask (g)
58.0784
68.0556
78.0387
87.9970 mass of water (g) (+0.0001g)
9.9517
9.9772
9.9831
9.9583 volume of water (ml) (+0.0001ml)
9.9786
10.0042
10.0101
9.9853
Purpose:
The purpose of the pipet calibration is to determine the accuracy and precision of 10ml of water at room temperature by using a 10ml volumetric pipet. Also, analyzing the analytical balance and the density of water from literature reference.
Experimental:
Description of glassware, equipments, and materials:
10 ml volumetric pipet (+0.02ml) Computer program: Excel oven
(1) 50 ml beaker Thermometer desiccator
(1) 250 ml beaker #1 stopper DI water
(1) 400 ml beaker 50 ml Erlenmeyer flask
Analytical balance (+0.0001g)
A clean 250 ml beaker was filled with 200ml of DI water and put aside to equilibrate to room temperature. Temperature was taken with a thermometer. A 50 ml Erlenmeyer flask was then cleaned rinsed and removed from water. The flask was marked with a pen and placed in oven to dry, then placed in desiccator to cool. The 10 ml volumetric pipet was cleaned with hot soapy water and rinsed with DI water before use. The mass of the empty flask with stopper was measured by analytical balance. A transfer quickly of 10 ml aliquot of DI water to flask replacing stopper; the mass was taken. The procedure was repeated three more times by adding additional 10 ml of water to flask and obtaining the mass after each addition.
Data Tables and Equations:
Summary Table 1: Mass and Volume of Water at 24⁰C
#1 #2 #3 #4 mi of flask (g)
48.1267
58.0784
68.0556
78.0387 mf of flask (g)
58.0784
68.0556
78.0387
87.9970 mass of water (g) (+0.0001g)
9.9517
9.9772
9.9831
9.9583 volume of water (ml) (+0.0001ml)
9.9786
10.0042
10.0101
9.9853
References: Lide, David R., Handbook of Chemistry and Physics. CRC Press: Boca Raton, FL, 84th ed. 2003/2004, 6-5. •Density of water at 24⁰C = 0.9972994 __p___ kg m-3