0610 S02 Qp 6

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Centre Number Candidate Name

Candidate Number

International General Certificate of Secondary Education

CAMBRIDGE INTERNATIONAL EXAMINATIONS

BIOLOGY
PAPER 6 Alternative to Practical

0610/6
MAY/JUNE SESSION 2002
1 hour

Candidates answer on the question paper. No additional materials are required.

TIME

1 hour

INSTRUCTIONS TO CANDIDATES Write your name, Centre number and candidate number in the spaces at the top of this page. Answer all questions. Write your answers in the spaces provided on the question paper. Use a sharp pencil for your drawings. Coloured pencils or crayons should not be used. INFORMATION FOR CANDIDATES The intended number of marks is given in brackets [ ] at the end of each question or part question.

FOR EXAMINER’S USE

1 2 3 4 TOTAL

This question paper consists of 10 printed pages and 2 blank pages. SP (CW/KN) S16792/3 © CIE 2002

[Turn over

2 1 A 15 cm length of Visking tubing was knotted at one end to make a small tube. A 10 cm3 sample of a mixture of active yeast in 2% sucrose solution was placed in the tube and the tube was knotted at the top. The tube was suspended in a small beaker containing limewater, as shown in Fig. 1.1. The apparatus was left for 30 minutes at room temperature (22 °C) and then observed.

For Examiner’s Use

limewater

active yeast in 2% sucrose solution Visking tubing Fig. 1.1 (a) (i) Suggest what changes you might expect to observe in the contents of the tube and the beaker. .................................................................................................................................. .................................................................................................................................. .................................................................................................................................. ..............................................................................................................................[3] (ii) State the metabolic process responsible for these changes. ..............................................................................................................................[1]

0610/6/M/J/02

3 (b) A similar apparatus was set up but the limewater was replaced by distilled water. The apparatus was left at room temperature for one hour. The contents of the Visking tubing and the beaker were tested using both the biuret test for protein and Benedict’s test for reducing sugar. The results of the biuret test are shown in Table 1.1 and the conclusions from the Benedict’s test are shown in Table 1.2. Table 1.1 colour after biuret test tube contents beaker contents purple blue tube contents beaker contents Table 1.2 conclusion after Benedict’s test reducing sugar present reducing sugar present

For Examiner’s Use

(i)

Suggest an explanation for the result of the biuret test on the contents of the beaker. .................................................................................................................................. .................................................................................................................................. ..............................................................................................................................[2]

(ii)

What colour would have been observed in the Benedict’s test for reducing sugar? ..............................................................................................................................[1]

(iii)

Suggest an explanation for the presence of reducing sugar in the beaker after one hour, when none was present in the original mixture of active yeast and sucrose. .....................................................................................................................................
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