Molecular Biology Ii

Topics: RNA polymerase, Promoter, DNA Pages: 4 (1260 words) Published: June 1, 2013
NAME:__________________________________ STUDENT#:______________________________ YORK UNIVERSITY FACULTY OF SCIENCE AND ENGINEERING SC/BIOL 3130N (3.0) – MOLECULAR BIOLOGY II MIDTERM I – January 31st, 2012, VERSION A INSTRUCTIONS: 1) Please sign the sign-in sheet that will be circulated during the exam. 2) Multiple choice – 12 questions, one mark each. Choose the best single mostcorrect answer and fill in all answers on the scantron sheet provided. Questions where more than one answer is selected will receive a mark of zero. Please be sure to write your name and shade in your student number on the scantron sheet. Please indicate on scantron sheet the version of your test. 3) Short answer questions – Answer four questions in the booklet provided. Point format/bullet format is permitted, if you prefer. If you write in pencil for these questions, no regrading will be permitted. Total 13 marks. 4) Total time – 75 minutes, from 11:35 to 12:50 pm. 5) No aids permitted.

Good Luck!

Multiple Choice (1 pt each). Pick the best answer: 1. Which of the following statements is/are false about mRNAs? a) b) c) d) e) The AUG is always downstream of +1 The ORF can contain introns. The poly-A tail is in the 3’UTR. The stop codon is always upstream of the terminator More than one of a-d are false

2. Immunoprecipitation is based on a similar principle as: a) b) c) d) e) SDS-PAGE Ion exchange chromatography Size exclusion chromatography Affinity chromatography PCR

3. How is a Western blot different from a Southern Blot, as we covered in class? a) b) c) d) e) f) A Western blot uses a gel. A Western blot uses a membrane. A Western blot uses an antibody. A Western blot uses chemilluminescence for detection More than one of a-d are different. None of a-d are different.

4. Which of the following statements about experiments we discussed testing for sigma subunit dissociation from RNA polymerase during transcription is/are false? a) Experiments we discussed using...
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