Venipuncture Equipment Review
Nneka Okoro
1. Explain how additives are identified in blood collection tubes.
By color of the tube top
2. List the 5 anticoagulants and their mode of action.
EDTA, sodium citrate and potassium oxalate bind calcium. heparin inhibits conversion of prothrombin to thrombin.
SPS (sodium polyethanol sulfonate) inhibits phagocytosis
3. Describe how gauge relates to the lumen of the needle.
The gauge indicates the diameter or lumen of the needle
4. Identify the lab departments that would require a heparin sample, EDTA sample, potassium oxalate sample, sodium polyethanol sulfate sample, sodium citrate, and acid citrate dextrose.
Heparin: Chemisty
EDTA: Hemtaology
Potassium Oxalate: Chemistry
Sodium Polyethanol Sulfate: Microbiology
Sodium Citrate: Coagulation
Acid Citrate Dextrose: Blood Bank
5. List and describe the parts of a needle.
Point: sharp end of the needle
Bevel: slanted end of the needle
Shaft: the longer slanted part
6. List the gauges of needles used for blood donations, normal adult draws, and pediatric draws.
Blood donations: 16 - 18
Adults: 20 – 21
Pediatric: 22 - 23
7. For drawing multiple tubes on a single patient, which system would be the best choice?
Evacuated Tube System
8. Complete blood clotting takes about how long?
30 – 60 minutes
9. Winged-infusion sets are to be used with what type of patient?
Pediatric or elderly patients
10. Where should you stick a patient when using a winged-infusion set?
Their hand
11. Describe serum, plasma, and whole blood.
Serum: fluid portion of bld after clotting used for chemistry testing such as glucose.
Plasma: fluid portion of bld containing an anticoagulant used for stat chemistry testing
Whole Blood: is mixed w/anticoagulant to prevent clotting used for hematology cell counts (CBCs)
12. Explain the purpose of the rubber sheath in a multi-sample needle.
To prevent blood from leaking into the holder
13. What is the factor that determines
1. Explain how additives are identified in blood collection tubes.
By color of the tube top
2. List the 5 anticoagulants and their mode of action.
EDTA, sodium citrate and potassium oxalate bind calcium. heparin inhibits conversion of prothrombin to thrombin.
SPS (sodium polyethanol sulfonate) inhibits phagocytosis
3. Describe how gauge relates to the lumen of the needle.
The gauge indicates the diameter or lumen of the needle
4. Identify the lab departments that would require a heparin sample, EDTA sample, potassium oxalate sample, sodium polyethanol sulfate sample, sodium citrate, and acid citrate dextrose.
Heparin: Chemisty
EDTA: Hemtaology
Potassium Oxalate: Chemistry
Sodium Polyethanol Sulfate: Microbiology
Sodium Citrate: Coagulation
Acid Citrate Dextrose: Blood Bank
5. List and describe the parts of a needle.
Point: sharp end of the needle
Bevel: slanted end of the needle
Shaft: the longer slanted part
6. List the gauges of needles used for blood donations, normal adult draws, and pediatric draws.
Blood donations: 16 - 18
Adults: 20 – 21
Pediatric: 22 - 23
7. For drawing multiple tubes on a single patient, which system would be the best choice?
Evacuated Tube System
8. Complete blood clotting takes about how long?
30 – 60 minutes
9. Winged-infusion sets are to be used with what type of patient?
Pediatric or elderly patients
10. Where should you stick a patient when using a winged-infusion set?
Their hand
11. Describe serum, plasma, and whole blood.
Serum: fluid portion of bld after clotting used for chemistry testing such as glucose.
Plasma: fluid portion of bld containing an anticoagulant used for stat chemistry testing
Whole Blood: is mixed w/anticoagulant to prevent clotting used for hematology cell counts (CBCs)
12. Explain the purpose of the rubber sheath in a multi-sample needle.
To prevent blood from leaking into the holder
13. What is the factor that determines