Viability of Microencapsulated Lactobacillus Acidophilus in Alginate Matrix During Exposure to Simulated Gastro - Intestinal Juice
Title: Viability of Microencapsulated Lactobacillus acidophilus in Alginate Matrix during exposure to Simulated Gastro - Intestinal Juice
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Abstract (English)
This investigation reports the effect of microencapsulation using different concentration of sodium alginate (1, 1,5, 2%) on the tolerance of probiotic Lactobacillus acidophilus under simulated gastrointestinal environments. Microencapsulation provided better protection at simulated conditions of gastric and bile salt. Higher surviving numbers of cells in AG 2% after incubation in gastric juice stimulated more cells to survive the sequential incubation into simulated intestinal juice and showed that the microencapsulation matrix was effective in protecting the entrapped cells with levels of survivors of 6.3 log cfu mL-1 compared to levels of 3.1 log cfu mL-1 for free cells, after 2 h in simulated intestinal juice. These studies demonstrated that microencapsulation of probiotic L. acidophilus in sodium alginate is an effective technique of protection under simulated gastrointestinal environment.
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Introduction
Lactic acid bacteria (LAB) are the organisms most commonly used as probiotics. Probiotic bacteria, lactic acid bacteria (LAB), which are typically associated with the human gastrointestinal tract, have been reported to suppress the growth of pathogens (Coconnier et al., 1993; Kaur, Chopra, & Saini, 2002; Lehto & Salminen, 1997; Lim, Huh, & Baek, 1993; Reid & Burton, 2002) and stabilize the digestive system by increasing intestinal barrier functions (Simon & Gorbach, 1984). Normally the stomach contains few bacteria (103 colony forming units per ml of gastric juice) whereas the bacterial concentration increases throughout the gut resulting in a final concentration in the colon of 1012 bacteria/g. Bacteria, forming the so-called resident intestinal microflora, do not normally
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Abstract (English)
This investigation reports the effect of microencapsulation using different concentration of sodium alginate (1, 1,5, 2%) on the tolerance of probiotic Lactobacillus acidophilus under simulated gastrointestinal environments. Microencapsulation provided better protection at simulated conditions of gastric and bile salt. Higher surviving numbers of cells in AG 2% after incubation in gastric juice stimulated more cells to survive the sequential incubation into simulated intestinal juice and showed that the microencapsulation matrix was effective in protecting the entrapped cells with levels of survivors of 6.3 log cfu mL-1 compared to levels of 3.1 log cfu mL-1 for free cells, after 2 h in simulated intestinal juice. These studies demonstrated that microencapsulation of probiotic L. acidophilus in sodium alginate is an effective technique of protection under simulated gastrointestinal environment.
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Introduction
Lactic acid bacteria (LAB) are the organisms most commonly used as probiotics. Probiotic bacteria, lactic acid bacteria (LAB), which are typically associated with the human gastrointestinal tract, have been reported to suppress the growth of pathogens (Coconnier et al., 1993; Kaur, Chopra, & Saini, 2002; Lehto & Salminen, 1997; Lim, Huh, & Baek, 1993; Reid & Burton, 2002) and stabilize the digestive system by increasing intestinal barrier functions (Simon & Gorbach, 1984). Normally the stomach contains few bacteria (103 colony forming units per ml of gastric juice) whereas the bacterial concentration increases throughout the gut resulting in a final concentration in the colon of 1012 bacteria/g. Bacteria, forming the so-called resident intestinal microflora, do not normally
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