Species Specific Dna Markers for Digitalis Purpurea L. (Scrophulariaceae) and Zanthoxylum Acanthopodium Dc.(Rutaceae)
ABSTRACT
Morphologically similar plants that are sometimes used as adulterants / substitutes for the prescribed drug usually causes reduced efficacy of plant based medicines / drug formulations. To minimize this problem, genotype specific DNA markers need to be developed. Remaining unchanged through short term variations in environment at different locations, and also through different phase of life cycle, DNA fingerprinting patterns constitute dependable DNA markers for ultimate individualization of a biological entity. DNA fingerprinting patterns in addition to supplementing drug assessment protocol as also establishing authentic taxonomic position are also important in establishing passport data in export of important plant genetic reserve and also resolving IPR conflicts. In this report DNA fingerprinting patterns of two medicinally important plants, widely known for their potential therapeutic value viz. Digitalis purpurea L. (Scrophulariaceae) and Zanthoxylum acanthopodium DC. (Rutaceae) have been presented.
Of the various methods developed for establishing genotype species markers,
1. Amplified Fragment Length Polymorphism (AFLP) analysis of genomes that, being done through stringent steps for providing highly specific fingerprinting pattern and
2. Randomly Amplified Polymorphic DNA (RAPD) that being cost effective, not requiring infrastructural and expertise input, provide a food comparative study when surveying large number of (wild) medicinal plants have been used.
Species specific AFLP patterns have been established for Zanthoxylum acanthopodiumd DC and Digitalis purpurea L. AFLP patterns of all the Zanthoxylum acanthopodium plant samples, collected from different areas in and around Meghalaya and Sikkim respectively were also found to be significantly similar. This establishes the suitability of using AFLP based DNA fingerprinting patterns for authentic characterization of plants.
Keywords- AFLP, Digitalis purpurea L,
Morphologically similar plants that are sometimes used as adulterants / substitutes for the prescribed drug usually causes reduced efficacy of plant based medicines / drug formulations. To minimize this problem, genotype specific DNA markers need to be developed. Remaining unchanged through short term variations in environment at different locations, and also through different phase of life cycle, DNA fingerprinting patterns constitute dependable DNA markers for ultimate individualization of a biological entity. DNA fingerprinting patterns in addition to supplementing drug assessment protocol as also establishing authentic taxonomic position are also important in establishing passport data in export of important plant genetic reserve and also resolving IPR conflicts. In this report DNA fingerprinting patterns of two medicinally important plants, widely known for their potential therapeutic value viz. Digitalis purpurea L. (Scrophulariaceae) and Zanthoxylum acanthopodium DC. (Rutaceae) have been presented.
Of the various methods developed for establishing genotype species markers,
1. Amplified Fragment Length Polymorphism (AFLP) analysis of genomes that, being done through stringent steps for providing highly specific fingerprinting pattern and
2. Randomly Amplified Polymorphic DNA (RAPD) that being cost effective, not requiring infrastructural and expertise input, provide a food comparative study when surveying large number of (wild) medicinal plants have been used.
Species specific AFLP patterns have been established for Zanthoxylum acanthopodiumd DC and Digitalis purpurea L. AFLP patterns of all the Zanthoxylum acanthopodium plant samples, collected from different areas in and around Meghalaya and Sikkim respectively were also found to be significantly similar. This establishes the suitability of using AFLP based DNA fingerprinting patterns for authentic characterization of plants.
Keywords- AFLP, Digitalis purpurea L,
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