Strawberries Investigation
AbstractThis experiment investigated the amount of DNA extracted from strawberries. This was done by using the independent variable of alcohol to affect the dependant variable of the amount of DNA extracted. This was done to find out if primary or secondary alcohol would create more DNA precipitate than the other. For this the primary alcohols used were; methanol and ethanol, and the secondary alcohol was; isopropyl. Of this the secondary alcohol, isopropyl was discovered to be the most effect alcohol to make DNA precipitate, as it produced the most amount of DNA. This investigation of extracting DNA is significant due to the study taken providing understanding and knowledge on DNA; this allows people to find out information of the cell structure,
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The main role of DNA acid is the long term storage of information. DNA is a set of blueprints or a code since it contains the instructions needed to construct other components of cells, such as proteins. The DNA divisions that carry this genetic information are called genes; other DNA sequences have different structural purposes, for example to create a body part, like an arm or a leg. Because of this, DNA provides information on hair colour, eye colour, skin colour, …show more content…
Also set up the filtering apparatus (see figure 1). Next blend 125g of Strawberries; Measure our 100mLs of the strawberry mixture and mix in 100mLs of distilled water, then put the mixture into the filtering apparatus, and leave to filter place filtrate mixture into a beaker. Fill a large beaker with hot water and place the smaller beaker inside (see figure 2) until the mixture is heated to 65°C. Take out the mixture and stir in 1gram of salt, 1g of meat tenderiser and 1mL of detergent. Pour 5mL of the now filtered and heated mixture into each of the 3 test tubes. In test tube 1add 5mL of methanol gently and slowly down the inside of the test tube. In test tube 2 add 5mL of ethanol. And in test tube 3 add 5mL of isopropyl. Allow for the DNA to precipitate and observe what is occurring. Once the DNA has precipitated it will look like cottony fluff in the alcohol or mixture. Collect the DNA using a wooden cocktail skewer, and place in labelled and sealed containers with 1mL of water and store in a fridge (at 4°C). Record each amount extracted. Repeat the method three times then run through a 1% agarose 1x TAE gel. All results observed should be recorded in a
The main role of DNA acid is the long term storage of information. DNA is a set of blueprints or a code since it contains the instructions needed to construct other components of cells, such as proteins. The DNA divisions that carry this genetic information are called genes; other DNA sequences have different structural purposes, for example to create a body part, like an arm or a leg. Because of this, DNA provides information on hair colour, eye colour, skin colour, …show more content…
Also set up the filtering apparatus (see figure 1). Next blend 125g of Strawberries; Measure our 100mLs of the strawberry mixture and mix in 100mLs of distilled water, then put the mixture into the filtering apparatus, and leave to filter place filtrate mixture into a beaker. Fill a large beaker with hot water and place the smaller beaker inside (see figure 2) until the mixture is heated to 65°C. Take out the mixture and stir in 1gram of salt, 1g of meat tenderiser and 1mL of detergent. Pour 5mL of the now filtered and heated mixture into each of the 3 test tubes. In test tube 1add 5mL of methanol gently and slowly down the inside of the test tube. In test tube 2 add 5mL of ethanol. And in test tube 3 add 5mL of isopropyl. Allow for the DNA to precipitate and observe what is occurring. Once the DNA has precipitated it will look like cottony fluff in the alcohol or mixture. Collect the DNA using a wooden cocktail skewer, and place in labelled and sealed containers with 1mL of water and store in a fridge (at 4°C). Record each amount extracted. Repeat the method three times then run through a 1% agarose 1x TAE gel. All results observed should be recorded in a