Review of Related Literature

Topics: Mosquito, Neem, Neem oil Pages: 10 (3684 words) Published: January 9, 2011
Research Larvicidal activity of neem oil (Azadirachta indica) formulation against mosquitoesVirendra K Dua1 , Akhilesh C Pandey1 , Kamaraju Raghavendra2 , Ashish Gupta1 , Trilochan Sharma1 and Aditya P Dash2 1  National Institute of Malaria Research, Field Unit, Sector-III, BHEL, Hardwar 249043, India2  National Institute of Malaria Research, 22 Sham Nath Marg, Delhi 110054, Indiaauthor email corresponding author emailMalaria Journal 2009, 8:124doi:10.1186/1475-2875-8-124The electronic version of this article is the complete one and can be found online at: http://www.malariajournal.com/content/8/1/124 Received:| 8 March 2009| Accepted:| 8 June 2009|

Published:| 8 June 2009|
© 2009 Dua et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.------------------------------------------------- AbstractBackgroundMosquitoes transmit serious human diseases, causing millions of deaths every year. Use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of botanical origin have been reported as useful for control of mosquitoes. Azadirachta indica (Meliaceae) and its derived products have shown a variety of insecticidal properties. The present paper discusses the larvicidal activity of neem-based biopesticide for the control of mosquitoes.MethodsLarvicidal efficacy of an emulsified concentrate of neem oil formulation (neem oil with polyoxyethylene ether, sorbitan dioleate and epichlorohydrin) developed by BMR & Company, Pune, India, was evaluated against late 3rd and early 4th instar larvae of different genera of mosquitoes. The larvae were exposed to different concentrations (0.5–5.0 ppm) of the formulation along with untreated control. Larvicidal activity of the formulation was also evaluated in field against Anopheles, Culex, and Aedes mosquitoes. The formulation was diluted with equal volumes of water and applied @ 140 mg a.i./m2 to different mosquito breeding sites with the help of pre calibrated knapsack sprayer. Larval density was determined at pre and post application of the formulation using a standard dipper.ResultsMedian lethal concentration (LC50) of the formulation against Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti was found to be 1.6, 1.8 and 1.7 ppm respectively. LC50 values of the formulation stored at 26°C, 40°C and 45°C for 48 hours against Ae. aegypti were 1.7, 1.7, 1.8 ppm while LC90 values were 3.7, 3.7 and 3.8 ppm respectively. Further no significant difference in LC50 and LC90 values of the formulation was observed against Ae. aegypti during 18 months storage period at room temperature. An application of the formulation at the rate of 140 mg a.i./m2 in different breeding sites under natural field conditions provided 98.1% reduction of Anopheles larvae on day 1; thereafter 100% reduction was recorded up to week 1 and more than 80% reduction up to week 3, while percent reduction against Culex larvae was 95.5% on day 1, and thereafter 80% reduction was achieved up to week 3. The formulation also showed 95.1% and, 99.7% reduction of Aedes larvae on day 1 and day 2 respectively; thereafter 100% larval control was observed up to day 7.ConclusionThe neem oil formulation was found effective in controlling mosquito larvae in different breeding sites under natural field conditions. As neem trees are widely distributed in India, their formulations may prove to be an effective and eco-friendly larvicide, which could be used as an alternative for malaria control.------------------------------------------------- BackgroundMosquitoes transmit serious human diseases like malaria, filariasis, Japanese encephalitis, dengue...

References: 1. Mittal PK, Subbarao SK: Prospects of using herbal products in the control of mosquito vectors. ICMR Bull 2003 , 33:1-10. Return to text 2. Brown AWA: Insecticide resistance in mosquitoes; a pragmatic review. J Am Mosq Control Assoc 1986 , 2:123-140. PubMed Abstract Return to text 3. Sukumar K, Perich MJ, Boobar LR: Botanical derivative in mosquito control: A Review. J Am Mosq Control Assoc 1991 , 7:210-237. PubMed Abstract Return to text 4. National Research Council: Neem: a tree for solving global problems. In Report of an adhoc panel of the Board on Science and Technology for International Development. National Academy Press, Washington, DC; 1992. Return to text 5. Isman MB: Botanical insecticides, deterrent and repellents in modern agriculture and an increasingly regulated world. Ann Rev Entomic 2006 , 51:45-66. Publisher Full Text Return to text 6. Schmutterer H: Properties of natural pesticides from the neem tree, Azadirachta indica. Ann Rev Entomol 1990 , 35:271-297. Publisher Full Text Return to text 7. Locantoni L, Guisti F, Cristofaro M, Pasqualini L, Esposito F, Lupetti P, Habluetzel A: Effect of neem extract on blood feeding oviposition and oocyte ultra structure in Anopheles stephensi Liston (Diptera: Culicidae). Tissue Cell 2006 , 38:361-371. PubMed Abstract | Publisher Full Text Return to text 8. Su T, Mulla MS: Antifeedancy of neem products containing Azadirachtin against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J Vector Ecol 1998 , 23:114-122. PubMed Abstract Return to text 9. Sharma VP, Dhiman RC: Neem oil as a sand fly (Diptera: Psychodidae) repellent. J Am Mosq Control Assoc 1993 , 9:364-366. PubMed Abstract Return to text 10. Schmutterer H: The neem tree (Azadirachta indica) and other Meliceous plants. In Source of Unique Natural Products for Integrated Pest Management, Medicine, Industry and other porposes. 1st edition. Mumbai: Neem Foundation; 2002. Return to text 11. Su T, Mulla MS: Ovicidal activity of neem products (azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera; Culicidae). J Am Mosq Control Assoc 1998 , 14:204-209. PubMed Abstract Return to text 12. World Health Organization: Instruction for determining the susceptibility or resistance of mosquito larvae. 1981. 13. Abbott WS: A method of computing the effectiveness of an insecticide. J Econ Entomol 1925 , 18:265-266. Return to text 14. World Health Organization: Chemical methods for the control of vector and pests of public health importance. Edited by: Chavassee DC, Yap NH. WHO; 1997. Return to text 15. Batra CP, Mittal PK, Adak T, Sharma VP: Efficacy of neem-water emulsion against mosquito immatures. Indian J Malariol 1998 , 35:15-21. PubMed Abstract Return to text 16. Rao DR, Reuben R, Venugopal MS, Nagasampgi BA, Schmutterer H: Evaluation of neem – Azadirachta indica with and without water management for the control of culicine mosquito larvae in rice field. Med Vet Entomol 1992 , 6:318-324. PubMed Abstract | Publisher Full Text Return to text 17. Dhar R, Dawar H, Garg SS, Basir F, Talwar GP: Effect of volatiles from neem and other natural products on gonotropic cycle and oviposition of Anopheles stephensi and An. culicifacies. J Med Entomol 1996 , 33:195-201. PubMed Abstract Return to text 18. Okumu FO, Knols BGJ, Fillinger U: Larvicidal effects of a neem (Azadirachta indica) oil formulation on the malaria vector Anopheles gambiae. Malar J 2007 , 6:63. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text Return to text 19. Gianotti RL, Bomblies A, Dafalla M, Issa-Arzika I, Duchemin JB, Eltahir EAB: Efficacy of local neem extracts for sustainable malaria vector control in an African village. Malar J 2008 , 7:138. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text Return to text 20. Zebit CPW: Effect of some crude and Azadirachta-enriched neem (Azadirachta indica) seed kernel extracts of larvae of Aedes aegypti. Entomol Exp Appl 1984 , 35:11-16. Publisher Full Text Return to text 21. Kreutzweiser DP: Non-target effects of neem based insecticides on aquatic invertebrates. Ecotoxicol Env Safety 1997 , 36:109-117. Publisher Full Text Return to text 22. Goektepe I, Portier R, Ahmedna M: Ecological risk assessment of neem based pesticides. J Env Sci Hlth B 2004 , 39:311-320. Publisher Full Text Return to text-------------------------------------------------
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