Rate of Diffusion of Mythelin Blue in Agar Jelly

Aim
To measure how different concentrations of mythelin blue affects the rate of diffusion through agar jelly.
Hypothesis
The diffusion of mythelin blue is directly proportional to its concentration, hence as the concentration increases; the rate of diffusion increases too.
Controlled Variables
Time time was kept constant while testing the diffusion spread of mythelin blue with each concentration.
Temperature the experiment was undertaken in room temperature as change in temperature can affect the rate of diffusion, as a higher/lower temperature would mean more/less kinetic energy in the molecules, which would affect the rate of diffusion.
Volume of mythelin blue the volume for each concentration was kept the same, as or else the solution could diffuse more or less depending.

Data
Table 1: Concentration of Mythelin Blue (%) | Diameter stain (mm, +/- 0.5mm) | | Trial 1 | Trial 2 | Trial 3 | Trial 4 | Trial 5 | | | | | | | 0.1 | 13 | 13 | 13 | 13 | 14 | 0.2 | 15 | 15 | 15 | 16 | 16 | 0.3 | 16 | 16 | 17 | 17 | 18 | 0.4 | 17 | 18 | 18 | 18 | 18 | 0.5 | 18 | 18 | 19 | 19 | 19 |

Table 2: Average | Std. Dev. | % Error | Confidence | Upper limit | Lower limit | | | | | | | 13.2 | 0.4 | 3.8 | 0.4 | 13.6 | 12.8 | 15.4 | 0.5 | 3.2 | 0.5 | 15.9 | 14.9 | 16.8 | 0.8 | 3.0 | 0.7 | 17.5 | 16.1 | 17.8 | 0.4 | 2.8 | 0.4 | 18.2 | 17.4 | 18.6 | 0.5 | 2.7 | 0.5 | 19.1 | 18.1 |

Calculation
Average:
(Trial 1 + trial 2 + trial 3 + trial 4 + trial 5) / 5 = Average 0.1 (13 + 13 + 13 + 13 + 14) / 5 = 13.2 0.2 (15 + 15 + 15 + 16 + 16) / 5 = 15.4 0.3 (16 + 16 + 17 + 17 + 18) / 5 = 16.8 0.4 (17 + 18 + 18 + 18 + 18) / 5 = 17.8 0.5 (18 + 18 + 19 + 19 + 19) / 5 = 18.6
Observation
During the data collection, the measuring of diffusion though the spread of mythelin blue in the agar jelly, there weren’t distinct lines of the spread, the edges of the stain were uneven and jaggy and hence the